Single nucleotide polymorphism analysis of the ITS2 region of two sympatric malaria mosquito species in Sweden : Anopheles daciaeandAnopheles messeae

• Four species of theAnopheles maculipenniscomplex have previously been recorded in Sweden. A recent addition to the complex isAnopheles daciae, which is considered to be closely related to, but distinct fromAnopheles messeae. The original designation ofAn. daciaewas based on five genetic differences (161, 165, 167, 362 and 382) in the second internal transcribed spacer (ITS) 2 of the ribosomal RNA. Further studies have shown that only two nucleotide differences (362 and 382) robustly separate the species. Thirty-threeAn. maculipenniscomplex mosquitoes were collected in the province of Uppland, Sweden. All wereAn. daciaebut showed double peaks for three variable positions (161, 165 and 167). When cloned, the intra-individual nucleotide variation was almost exclusively fixed with either TTC or AAT, originally diagnostic forAn. messaeandAn. daciae, respectively. To further investigate the intra-individual variation, nineAn. daciaeand 11An. messeaewere collected in southern Sweden and their ITS2 fragments were amplified and sequenced using Illumina MiSeq sequencing (Illumina, Inc., San Diego, CA, USA). For the diagnostic nucleotide 382 no intra-individual variation could be detected. However, although eachAn. daciaespecimen carried several ITS2 sequence variants for the four other nucleotides, there was no intra-individual variation in theAn. messeaespecimens.

Subject headings

NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

NATURVETENSKAP  -- Biologi -- Zoologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Zoology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Keyword

Anopheles maculipennis complex

species diagnosis

intra-individual variation

haplotypes

polymorphic

Publication and Content Type

ref (subject category)

art (subject category)