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Single-cell analysi...
Single-cell analysis uncovers fibroblast heterogeneity and criteria for fibroblast and mural cell identification and discrimination
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- Muhl, Lars (author)
- Karolinska Institutet
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- Genove, Guillem (author)
- Karolinska Institutet
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- Leptidis, Stefanos (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Liu, Jianping (author)
- Karolinska Institutet
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- He, Liqun (author)
- Uppsala universitet,Vaskulärbiologi,Tianjin Med Univ, Gen Hosp, Dept Neurosurg,Minist Educ & Tianjin City, Tianjin Neurol Inst,Key Lab PostNeuroinjury Neuro, Tianjin 300052, Peoples R China.
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- Mocci, Giuseppe (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Sun, Ying (author)
- Uppsala universitet,Vaskulärbiologi
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- Gustafsson, Sonja (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Buyandelger, Byambajav (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Chivukula, Indira, V (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Segerstolpe, Asa (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.;Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA USA.
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- Raschperger, Elisabeth (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Hansson, Emil M. (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Bjorkegren, Johan L. M. (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.;Icahn Sch Med Mt Sinai, Icahn Inst Genom & Multiscale Biol, Dept Genet & Genom Sci, One Gustave L Levy Pl, New York, NY 10029 USA.
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- Peng, Xiao-Rong (author)
- AstraZeneca, Metab Res & Early Dev, Biosci Metab, Cardiovasc Renal & Metab CVRM BioPharmaceut R&D, Gothenburg, Sweden.
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- Vanlandewijck, Michael, 1982- (author)
- Uppsala universitet,Vaskulärbiologi,Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.
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- Lendahl, Urban (author)
- Karolinska Institutet
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- Betsholtz, Christer (author)
- Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden.;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden.;Tianjin Med Univ, Gen Hosp, Dept Neurosurg,Minist Educ & Tianjin City, Tianjin Neurol Inst,Key Lab PostNeuroinjury Neuro, Tianjin 300052, Peoples R China.
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Karolinska Institutet Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr, Blickagangen 6, SE-14157 Huddinge, Sweden;Karolinska Inst, Dept Med Huddinge, SE-14157 Huddinge, Sweden. (creator_code:org_t)
- 2020-08-07
- 2020
- English.
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In: Nature Communications. - : Springer Nature. - 2041-1723. ; 11:1
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Abstract
Subject headings
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- Many important cell types in adult vertebrates have a mesenchymal origin, including fibroblasts and vascular mural cells. Although their biological importance is undisputed, the level of mesenchymal cell heterogeneity within and between organs, while appreciated, has not been analyzed in detail. Here, we compare single-cell transcriptional profiles of fibroblasts and vascular mural cells across four murine muscular organs: heart, skeletal muscle, intestine and bladder. We reveal gene expression signatures that demarcate fibroblasts from mural cells and provide molecular signatures for cell subtype identification. We observe striking inter- and intra-organ heterogeneity amongst the fibroblasts, primarily reflecting differences in the expression of extracellular matrix components. Fibroblast subtypes localize to discrete anatomical positions offering novel predictions about physiological function(s) and regulatory signaling circuits. Our data shed new light on the diversity of poorly defined classes of cells and provide a foundation for improved understanding of their roles in physiological and pathological processes. To define and distinguish fibroblasts from vascular mural cells have remained challenging. Here, using single-cell RNA sequencing and tissue imaging, the authors provide a molecular basis for cell type classification and reveal inter- and intra-organ diversity of these cell types.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Muhl, Lars
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Genove, Guillem
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Leptidis, Stefan ...
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Liu, Jianping
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He, Liqun
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Mocci, Giuseppe
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show more...
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Sun, Ying
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Gustafsson, Sonj ...
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Buyandelger, Bya ...
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Chivukula, Indir ...
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Segerstolpe, Asa
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Raschperger, Eli ...
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Hansson, Emil M.
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Bjorkegren, Joha ...
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Peng, Xiao-Rong
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Vanlandewijck, M ...
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Lendahl, Urban
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Betsholtz, Chris ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Nature Communica ...
- By the university
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Uppsala University
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Karolinska Institutet