Search: onr:"swepub:oai:DiVA.org:uu-421690" >
MiR-155 is overexpr...
MiR-155 is overexpressed in patients with atopic dermatitis and modulates T-cell proliferative responses by targeting cytotoxic T lymphocyte-associated antigen 4.
-
- Sonkoly, Enikö (author)
- Karolinska Institutet
-
Janson, Peter (author)
-
Majuri, Marja-Leena (author)
-
show more...
-
Savinko, Terhi (author)
-
Fyhrquist, Nanna (author)
-
- Eidsmo, Liv (author)
- Karolinska Institutet
-
- Xu, Ning (author)
- Karolinska Institutet
-
- Meisgen, Florian (author)
- Karolinska Institutet
-
Wei, Tianling (author)
-
- Bradley, Maria (author)
- Karolinska Institutet
-
Stenvang, Jan (author)
-
Kauppinen, Sakari (author)
-
Alenius, Harri (author)
-
Lauerma, Antti (author)
-
Homey, Bernhard (author)
-
- Winqvist, Ola (author)
- Karolinska Institutet
-
- Ståhle, Mona (author)
- Karolinska Institutet
-
- Pivarcsi, Andor (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- English.
-
In: Journal of Allergy and Clinical Immunology. - : Elsevier BV. - 0091-6749 .- 1097-6825. ; 126:3, s. 581-9.e1
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- BACKGROUND: MicroRNAs (miRNAs) are short noncoding RNAs that suppress gene expression at the posttranscriptional level. Atopic dermatitis is a common chronic inflammatory skin disease characterized by the presence of activated T cells within the skin.OBJECTIVE: We sought to explore the role of miRNAs in the pathogenesis of atopic dermatitis.METHODS: Global miRNA expression in healthy and lesional skin of patients with atopic dermatitis was compared by using TaqMan MicroRNA Low Density Arrays. miR-155 expression in tissues and cells was quantified by means of quantitative real-time PCR. The cellular localization of miR-155 was analyzed by means of in situ hybridization. The regulation of cytotoxic T lymphocyte-associated antigen (CTLA-4) by miR-155 was investigated by using luciferase reporter assays and flow cytometry. CTLA-4 expression and functional assays were performed on T(H) cells overexpressing miR-155.RESULTS: miR-155 was one of the highest-ranked upregulated miRNAs in patients with atopic dermatitis. In the skin miR-155 was predominantly expressed in infiltrating immune cells. miR-155 was upregulated during T-cell differentiation/activation and was markedly induced by T-cell activators in PBMCs in vitro and by superantigens and allergens in the skin in vivo. CTLA-4, an important negative regulator of T-cell activation, was identified as a direct target of miR-155. Overexpression of miR-155 in T(H) cells resulted in decreased CTLA-4 levels accompanied by an increased proliferative response.CONCLUSION: miR-155 is significantly overexpressed in patients with atopic dermatitis and might contribute to chronic skin inflammation by increasing the proliferative response of T(H) cells through the downregulation of CTLA-4.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Sonkoly, Enikö
-
Janson, Peter
-
Majuri, Marja-Le ...
-
Savinko, Terhi
-
Fyhrquist, Nanna
-
Eidsmo, Liv
-
show more...
-
Xu, Ning
-
Meisgen, Florian
-
Wei, Tianling
-
Bradley, Maria
-
Stenvang, Jan
-
Kauppinen, Sakar ...
-
Alenius, Harri
-
Lauerma, Antti
-
Homey, Bernhard
-
Winqvist, Ola
-
Ståhle, Mona
-
Pivarcsi, Andor
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Immunology in th ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Dermatology and ...
- Articles in the publication
-
Journal of Aller ...
- By the university
-
Uppsala University
-
Karolinska Institutet