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Identification and characterization of a novel, psoriasis susceptibility-related noncoding RNA gene, PRINS.
- Article/chapterEnglish2005
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LIBRIS-ID:oai:DiVA.org:uu-421711
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-421711URI
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https://doi.org/10.1074/jbc.M501704200DOI
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Language:English
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Summary in:English &language:-1_t
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Subject category:art swepub-publicationtype
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To identify genetic factors contributing to psoriasis susceptibility, gene expression profiles of uninvolved epidermis from psoriatic patients and epidermis from healthy individuals were compared. Besides already characterized genes, we identified a cDNA with yet unknown functions, which we further characterized and named PRINS (Psoriasis susceptibility-related RNA Gene Induced by Stress). In silico structural and homology studies suggested that PRINS may function as a noncoding RNA. PRINS harbors two Alu elements, it is transcribed by RNA polymerase II, and it is expressed at different levels in various human tissues. Real time reverse transcription-PCR analysis showed that PRINS was expressed higher in the uninvolved epidermis of psoriatic patients compared with both psoriatic lesional and healthy epidermis, suggesting a role for PRINS in psoriasis susceptibility. PRINS is regulated by the proliferation and differentiation state of keratinocytes. Treatment with T-lymphokines, known to precipitate psoriatic symptoms, decreased PRINS expression in the uninvolved psoriatic but not in healthy epidermis. Real time reverse transcription-PCR analysis showed that stress signals such as ultraviolet-B irradiation, viral infection (herpes simplex virus), and translational inhibition increased the RNA level of PRINS. Gene-specific silencing of PRINS by RNA interference revealed that down-regulation of PRINS impairs cell viability after serum starvation but not under normal serum conditions. Our findings suggest that PRINS functions as a noncoding regulatory RNA, playing a protective role in cells exposed to stress. Furthermore, elevated PRINS expression in the epidermis may contribute to psoriasis susceptibility.
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Bata-Csorgo, Zsuzsanna
(author)
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Pivarcsi, Andor
(author)
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Polyanka, Hilda
(author)
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Kenderessy-Szabo, Anna
(author)
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Molnar, Gergely
(author)
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Szentpali, Karoly
(author)
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Bari, Lilla
(author)
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Megyeri, Klara
(author)
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Mandi, Yvette
(author)
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Dobozy, Attila
(author)
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Kemeny, Lajos
(author)
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Szell, Marta
(author)
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In:Journal of Biological Chemistry280:25, s. 24159-670021-92581083-351X
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Sonkoly, Eniko
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Bata-Csorgo, Zsu ...
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Pivarcsi, Andor
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Polyanka, Hilda
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Kenderessy-Szabo ...
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Molnar, Gergely
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Szentpali, Karol ...
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Bari, Lilla
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Megyeri, Klara
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Mandi, Yvette
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Dobozy, Attila
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Kemeny, Lajos
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Szell, Marta
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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Uppsala University