Search: onr:"swepub:oai:DiVA.org:uu-428751" >
Dynamics of DNA Clo...
Dynamics of DNA Clogging in Hafnium Oxide Nanopores
-
- Li, Shiyu (author)
- Uppsala universitet,Fasta tillståndets elektronik,Uppsala University,Shiyu Li
-
- Zeng, Shuangshuang (author)
- Uppsala universitet,Fasta tillståndets elektronik
-
- Wen, Chenyu (author)
- Uppsala universitet,Fasta tillståndets elektronik
-
show more...
-
- Barbe, Laurent (author)
- Uppsala universitet,Mikrosystemteknik
-
- Tenje, Maria (author)
- Uppsala universitet,Mikrosystemteknik
-
- Zhang, Zhen, 1979- (author)
- Uppsala universitet,Fasta tillståndets elektronik
-
- Hjort, Klas, 1964- (author)
- Uppsala universitet,Mikrosystemteknik
-
- Zhang, Shi-Li (author)
- Uppsala universitet,Fasta tillståndets elektronik
-
show less...
-
(creator_code:org_t)
- 2020-12-14
- 2020
- English.
-
In: Journal of Physical Chemistry B. - : American Chemical Society (ACS). - 1520-6106 .- 1520-5207. ; 124:51, s. 11573-11583
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://pubs.acs.org...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Interfacing solid-state nanopores with biological systems has been exploited as a versatile analytical platform for analysis of individual biomolecules. Although clogging of solid-state nanopores due to nonspecific interactions between analytes and pore walls poses a persistent challenge in attaining the anticipated sensing efficacy, insufficient studies focus on elucidating the clogging dynamics. Herein, we investigate the DNA clogging behavior by passing double-stranded (ds) DNA molecules of different lengths through hafnium oxide(HfO2)-coated silicon (Si) nanopore arrays, at different bias voltages and electrolyte pH values. Employing stable and photoluminescent-free HfO2/Si nanopore arrays permits a parallelized visualization of DNA clogging with confocal fluorescence microscopy. We find that the probability of pore clogging increases with both DNA length and bias voltage. Two types of clogging are discerned: persistent and temporary. In the time-resolved analysis, temporary clogging events exhibit a shorter lifetime at higher bias voltage. Furthermore, we show that the surface charge density has a prominent effect on the clogging probability because of electrostatic attraction between the dsDNA and the HfO2 pore walls. An analytical model based on examining the energy landscape along the DNA translocation trajectory is developed to qualitatively evaluate the DNA–pore interaction. Both experimental and theoretical results indicate that the occurrence of clogging is strongly dependent on the configuration of translocating DNA molecules and the electrostatic interaction between DNA and charged pore surface. These findings provide a detailed account of the DNA clogging phenomenon and are of practical interest for DNA sensing based on solid-state nanopores.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Li, Shiyu
-
Zeng, Shuangshua ...
-
Wen, Chenyu
-
Barbe, Laurent
-
Tenje, Maria
-
Zhang, Zhen, 197 ...
-
show more...
-
Hjort, Klas, 196 ...
-
Zhang, Shi-Li
-
show less...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
-
ENGINEERING AND ...
-
and Electrical Engin ...
-
and Other Electrical ...
- Articles in the publication
-
Journal of Physi ...
- By the university
-
Uppsala University