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Brush Samples of Or...
Brush Samples of Oral Lesions to FTA Elute Card for High-risk Human Papilloma Virus Diagnosis
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- Runow Stark, Christina (author)
- Uppsala universitet,Käkkirurgi,Folktandvården Stockholms län AB
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- Gustavsson, Inger (author)
- Uppsala universitet,Medicinsk genetik och genomik
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Horal, Peter (author)
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- Kotopouli, Maria (author)
- Karolinska Institutet
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- Gyllensten, Ulf (author)
- Uppsala universitet,Medicinsk genetik och genomik
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- Hirsch, Jan-Michaél (author)
- Uppsala universitet,Käkkirurgi,Folktandvården Stockholms län AB
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(creator_code:org_t)
- 2021-01-08
- 2021
- English.
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In: Anticancer Research. - : Anticancer Research USA Inc.. - 0250-7005 .- 1791-7530. ; 41:1, s. 269-277
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Abstract
Subject headings
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- AIM: To investigate the level of agreement between three non-invasive methods for hrHPV diagnosis in oral and oropharyngeal squamous cell carcinoma (OSCC, OPSCC) and in oral mucosal lesions.MATERIALS AND METHODS: For hrHPV DNA FTA Elute card™ and Anyplex II HPV28™ were used and for hrHPV mRNA PreTect SEE™ in tumour patients (n=60), non-tumour lesions (n=51), immunosuppression or previous hrHPV-infection (n=32).RESULTS: The level of agreement between the DNA-methods was 82.2% (k=0.54, p=0.001). Pair-wise comparison for the FTA Elute card were close to the reference (AUC=0.83, 95% CI=0.73-0.90). hrHPV mRNA was diagnosed in 50% of the tumours, with an agreement level of 58.3%, compared to Anyplex II (k=0.17, p=0.04). The hrHPV positivity in oral lesions was 3.9% for immunosuppression and for previous HPV infection 9.4%.CONCLUSION: The FTA card is reliable for hrHPV DNA diagnosis while mRNA gives an insight into viral activity and correlates with severity of the lesion.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- DNA
- FTA Elute card
- hrHPV
- human papillomavirus
- mRNA
- oral- and oropharyngeal cancer
- potentially malignant oral disorders
- real-time PCR
Publication and Content Type
- ref (subject category)
- art (subject category)
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