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Label-Free Optical ...
Label-Free Optical Detection of DNA Polymerase Docking on Solid-State Nanopore Arrays
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- Li, Shiyu (author)
- Uppsala universitet,Fasta tillståndets elektronik
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- Zeng, Shuangshuang (author)
- Uppsala universitet,Fasta tillståndets elektronik
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- Wen, Chenyu (author)
- Uppsala universitet,Fasta tillståndets elektronik
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- Zhang, Zhen, 1979- (author)
- Uppsala universitet,Fasta tillståndets elektronik
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- Hjort, Klas, 1964- (author)
- Uppsala universitet,Mikrosystemteknik
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- Zhang, Shi-Li (author)
- Uppsala universitet,Fasta tillståndets elektronik
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- Integration of motor enzymes with biological nanopores has enabled commercial DNA sequencing, yet studies of the similar principle applying to solid-state nanopores are limited. Here, we demonstrate the label-free optical detection of phi29 DNA polymerase (DNAP) docking onto truncated-pyramidal nanopores (TPP). Atomic layer deposition of hafnium oxide is employed to shrink the pore opening size of original silicon (Si) TPP to sub-10 nm. Ionic current measurements of single TPP of an opening size comparable to DNAP show that polymerase-DNA complexes can temporally dock onto the TPP with a certain docking orientation, while the majority are translocation events. A label-free optical detection method using Ca2+ sensitive dye is employed to detect the docking of DNAP on 5-by-5 nanopore arrays. The results of the proof-of-concept experiment show that this label-free detection strategy is capable of accessing the docking events of DNAP on solid-state nanopore arrays.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Publication and Content Type
- vet (subject category)
- ovr (subject category)
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