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System-wide identif...
System-wide identification and prioritization of enzyme substrates by thermal analysis
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- Saei, Amir Ata (author)
- Karolinska Institutet
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- Beusch, Christian M. (author)
- Karolinska Institutet
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- Sabatier, Pierre (author)
- Karolinska Institutet
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Astorga Wells, Juan (author)
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- Gharibi, Hassan (author)
- Karolinska Institutet
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Meng, Zhaowei (author)
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Chernobrovkin, Alexey (author)
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- Rodin, Sergey (author)
- Uppsala universitet,Thoraxkirurgi,Division of Physiological Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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Näreoja, Katja (author)
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Thorsell, Ann-Gerd (author)
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Karlberg, Tobias (author)
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Cheng, Qing (author)
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- Lundström, Susanna L. (author)
- Karolinska Institutet
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Gaetani, Massimiliano (author)
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- Végvári, Ákos (author)
- Karolinska Institutet
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- Arnér, Elias S. J. (author)
- Karolinska Institutet
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- Schüler, Herwig (author)
- Karolinska Institutet
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- Zubarev, Roman A. (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2021-02-26
- 2021
- English.
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In: Nature Communications. - : Springer Nature. - 2041-1723. ; 12:1
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Abstract
Subject headings
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- Despite the immense importance of enzyme-substrate reactions, there is a lack of general and unbiased tools for identifying and prioritizing substrate proteins that are modified by the enzyme on the structural level. Here we describe a high-throughput unbiased proteomics method called System-wide Identification and prioritization of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that the enzymatic post-translational modification of substrate proteins is likely to change their thermal stability. In our proof-of-concept studies, SIESTA successfully identifies several known and novel substrate candidates for selenoprotein thioredoxin reductase 1, protein kinase B (AKT1) and poly-(ADP-ribose) polymerase-10 systems. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, opening opportunities to investigate the effect of post-translational modifications on signal transduction and facilitate drug discovery.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Saei, Amir Ata
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Beusch, Christia ...
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Sabatier, Pierre
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Astorga Wells, J ...
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Gharibi, Hassan
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Meng, Zhaowei
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show more...
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Chernobrovkin, A ...
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Rodin, Sergey
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Näreoja, Katja
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Thorsell, Ann-Ge ...
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Karlberg, Tobias
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Cheng, Qing
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Lundström, Susan ...
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Gaetani, Massimi ...
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Végvári, Ákos
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Arnér, Elias S. ...
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Schüler, Herwig
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Zubarev, Roman A ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Nature Communica ...
- By the university
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Uppsala University
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Karolinska Institutet