SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:uu-437495"
 

Search: onr:"swepub:oai:DiVA.org:uu-437495" > Structure and mecha...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Structure and mechanism of a phage-encoded SAM lyase revises catalytic function of enzyme family

Guo, Xiaohu (author)
Uppsala universitet,Institutionen för cell- och molekylärbiologi
Söderholm, Annika (author)
Uppsala universitet,Strukturbiologi
Kanchugal Puttaswamy, Sandesh (author)
Uppsala universitet,Strukturbiologi
show more...
Isaksen, Geir V (author)
Uppsala universitet,Institutionen för cell- och molekylärbiologi,UiT Arctic Univ Norway, Hylleraas Ctr Quantum Mol Sci, Dept Chem, Tromso, Norway
Warsi, Omar M. (author)
Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
Eckhard, Ulrich (author)
Uppsala universitet,Institutionen för cell- och molekylärbiologi
Trigüis, Silvia (author)
Uppsala universitet,Strukturbiologi
Gogoll, Adolf, 1957- (author)
Uppsala universitet,Organisk kemi
Jerlstrom-Hultqvist, Jon, 1982- (author)
Uppsala universitet,Mikrobiologi,Institutionen för medicinsk biokemi och mikrobiologi
Åqvist, Johan (author)
Uppsala universitet,Beräkningsbiologi och bioinformatik
Andersson, Dan I. (author)
Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
Selmer, Maria (author)
Uppsala universitet,Strukturbiologi
show less...
 (creator_code:org_t)
eLife Sciences Publications Ltd, 2021
2021
English.
In: eLIFE. - : eLife Sciences Publications Ltd. - 2050-084X. ; 10
Related links:
https://doi.org/10.7...
show more...
https://uu.diva-port... (primary) (Raw object)
https://urn.kb.se/re...
https://doi.org/10.7...
show less...
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • The first S-adenosyl methionine (SAM) degrading enzyme (SAMase) was discovered in bacteriophage T3, as a counter-defense against the bacterial restriction-modification system, and annotated as a SAM hydrolase forming 5’-methyl-thioadenosine (MTA) and L-homoserine. From environmental phages, we recently discovered three SAMases with barely detectable sequence similarity to T3 SAMase and without homology to proteins of known structure. Here, we present the very first phage SAMase structures, in complex with a substrate analogue and the product MTA. The structure shows a trimer of alpha–beta sandwiches similar to the GlnB-like superfamily, with active sites formed at the trimer interfaces. Quantum-mechanical calculations, thin-layer chromatography, and nuclear magnetic resonance spectroscopy demonstrate that this family of enzymes are not hydrolases but lyases forming MTA and L-homoserine lactone in a unimolecular reaction mechanism. Sequence analysis and in vitro and in vivo mutagenesis support that T3 SAMase belongs to the same structural family and utilizes the same reaction mechanism.

Subject headings

NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

Biologi med inriktning mot strukturbiologi
Biology with specialization in Structural Biology

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

  • eLIFE (Search for host publication in LIBRIS)

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view