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Mass spectrometry i...
Mass spectrometry imaging reveals brain-region specific changes in metabolism and acetylcholine levels in experimental Parkinson’s disease and L-DOPA-induced dyskinesia
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- Fridjonsdottir, Elva (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Medical mass spectrometry imaging
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- Vallianatou, Theodosia (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Aerts, Jordan T. (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Medical mass spectrometry imaging
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- Mantas, Ioannis (author)
- Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
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- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab,Medical mass spectrometry imaging
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- Shariatgorji, Mohammadreza (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab,Medical mass spectrometry imaging
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- Jansson, Erik T. (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Medical mass spectrometry imaging
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- Svenningsson, Per (author)
- Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
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- Bezard, Erwan (author)
- Université de Bordeaux, Institut des Maladies Neurodégénératives, Bordeaux, France.; CNRS, Institut des Maladies Neurodégénératives, Bordeaux, France.; Motac Neuroscience, Manchester, M15 6WE, United Kingdom.
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- Andrén, Per E., Professor, 1957- (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
Close
- There is evidence that cholinergic alterations are linked to various motor and non-motor symptoms of Parkinson’s disease. We therefore used mass spectrometry imaging to investigate regional changes in acetylcholine abundance in the brain of a non-human primate model of Parkinson’s disease (PD) and L-DOPA-induced dyskinesia (LID). We also present an experimental design for performing untargeted analysis using MALDI-MSI with multiple experiments incorporating quality control samples to monitor experimental variability. We observed that MPTP treatment (i) led to reductions in putaminal acetylcholine levels that persisted after L-DOPA treatment and (ii) appeared to induce a shift of choline metabolism from α-glycerophosphocholine towards betaine. LID animals exhibited reduced levels of various metabolites important for brain homeostasis including S-adenosylmethionine, glutathione, adenosine monophosphate, and acylcarnitines. The vasculature marker heme B was upregulated in the putamen of LID animals, suggesting increased blood-flow in the dyskinetic putamen. These results provide new insights into pathological choline-related metabolic changes in PD and LID.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Acetylcholine
- Parkinson's disease
- L-DOPA-induced dyskinesia
- MALDI
- Mass spectrometry imaging
- Farmaceutisk vetenskap
- Pharmaceutical Science
Publication and Content Type
- vet (subject category)
- ovr (subject category)
To the university's database
- By the author/editor
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Fridjonsdottir, ...
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Vallianatou, The ...
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Aerts, Jordan T.
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Mantas, Ioannis
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Nilsson, Anna
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Shariatgorji, Mo ...
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show more...
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Jansson, Erik T.
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Svenningsson, Pe ...
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Bezard, Erwan
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Andrén, Per E., ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- By the university
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Uppsala University