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Optofluidic Raman-a...
Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions
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- Lee, Kang Soo (author)
- Swiss Fed Inst Technol, Dept Civil Environm & Geomat Engn, Inst Environm Engn, Zurich, Switzerland.
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- Pereira, Fatima C. (author)
- Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Dept Microbiol & Ecosyst Sci, Vienna, Austria.
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- Palatinszky, Marton (author)
- Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Dept Microbiol & Ecosyst Sci, Vienna, Austria.
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- Behrendt, Lars (author)
- Uppsala universitet,Miljötoxikologi,Science for Life Laboratory, SciLifeLab
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- Alcolombri, Uria (author)
- Swiss Fed Inst Technol, Dept Civil Environm & Geomat Engn, Inst Environm Engn, Zurich, Switzerland.
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- Berry, David (author)
- Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Dept Microbiol & Ecosyst Sci, Vienna, Austria.;Univ Vienna, Med Univ Vienna, Joint Microbiome Facil, Vienna, Austria.
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- Wagner, Michael (author)
- Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Dept Microbiol & Ecosyst Sci, Vienna, Austria.;Aalborg Univ, Ctr Microbial Communities, Dept Chem & Biosci, Aalborg, Denmark.
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- Stocker, Roman (author)
- Swiss Fed Inst Technol, Dept Civil Environm & Geomat Engn, Inst Environm Engn, Zurich, Switzerland.
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Swiss Fed Inst Technol, Dept Civil Environm & Geomat Engn, Inst Environm Engn, Zurich, Switzerland Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Dept Microbiol & Ecosyst Sci, Vienna, Austria. (creator_code:org_t)
- 2020-12-11
- 2021
- English.
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In: Nature Protocols. - : Springer Nature. - 1754-2189 .- 1750-2799. ; 16:2
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Stable isotope labeling of microbial taxa of interest and their sorting provide an efficient and direct way to answer the question "who does what?" in complex microbial communities when coupled with fluorescence in situ hybridization or downstream 'omics' analyses. We have developed a platform for automated Raman-based sorting in which optical tweezers and microfluidics are used to sort individual cells of interest from microbial communities on the basis of their Raman spectra. This sorting of cells and their downstream DNA analysis, such as by mini-metagenomics or single-cell genomics, or cultivation permits a direct link to be made between the metabolic roles and the genomes of microbial cells within complex microbial communities, as well as targeted isolation of novel microbes with a specific physiology of interest. We describe a protocol from sample preparation through Raman-activated live cell sorting. Subsequent cultivation of sorted cells is described, whereas downstream DNA analysis involves well-established approaches with abundant methods available in the literature. Compared with manual sorting, this technique provides a substantially higher throughput (up to 500 cells per h). Furthermore, the platform has very high sorting accuracy (98.3 +/- 1.7%) and is fully automated, thus avoiding user biases that might accompany manual sorting. We anticipate that this protocol will empower in particular environmental and host-associated microbiome research with a versatile tool to elucidate the metabolic contributions of microbial taxa within their complex communities. After a 1-d preparation of cells, sorting takes on the order of 4 h, depending on the number of cells required. This protocol describes a microfluidic platform for high-throughput sorting of individual cells from microbial communities. Metabolically active cells are labeled using D2O or C-13, selected by Raman imaging, and sorted on-chip with optical tweezers.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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