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A Long-Term Conserv...
A Long-Term Conserved Satellite DNA That Remains Unexpanded in Several Genomes of Characiformes Fish Is Actively Transcribed
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- dos Santos, Rodrigo Zeni (author)
- Univ Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, UNESP, Campus Bauru, Bauru, SP, Brazil.
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- Calegari, Rodrigo Milan (author)
- Univ Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, UNESP, Campus Bauru, Bauru, SP, Brazil.
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- de Andrade Silva, Duilio Mazzoni Zerbinato (author)
- Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Biol Estrutural & Func, UNESP, Botucatu, SP, Brazil.
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- Ruiz-Ruano, Francisco J. (author)
- Uppsala universitet,Systematisk biologi
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- Melo, Silvana (author)
- Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Biol Estrutural & Func, UNESP, Botucatu, SP, Brazil.
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- Oliveira, Claudio (author)
- Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Biol Estrutural & Func, UNESP, Botucatu, SP, Brazil.
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- Foresti, Fausto (author)
- Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Biol Estrutural & Func, UNESP, Botucatu, SP, Brazil.
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- Uliano-Silva, Marcela (author)
- Wellcome Sanger Inst, Cambridge, England.
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- Porto-Foresti, Fabio (author)
- Univ Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, UNESP, Campus Bauru, Bauru, SP, Brazil.
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- Utsunomia, Ricardo (author)
- Univ Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, UNESP, Campus Bauru, Bauru, SP, Brazil.;Univ Fed Rural Rio de Janeiro, Inst Ciencias Biol & Saude, Dept Genet, Seropedica, RJ, Brazil.
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Univ Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, UNESP, Campus Bauru, Bauru, SP, Brazil Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Biol Estrutural & Func, UNESP, Botucatu, SP, Brazil. (creator_code:org_t)
- 2021-01-27
- 2021
- English.
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In: Genome Biology and Evolution. - : Oxford University Press. - 1759-6653. ; 13:2
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Abstract
Subject headings
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- Eukaryotic genomes contain large amounts of repetitive DNA sequences, such as tandemly repeated satelliteDNAs (satDNAs). These sequences are highly dynamic and tend to begenus- or species-specific due to their particular evolutionary pathways, although there are few unusual cases of conserved satDNAs over long periods of time. Here, we used multiple approaches to reveal that an satDNA named CharSat01-52 originated in the last common ancestor of Characoidei fish, a superfamily within the Characiformes order, similar to 140-78Ma, whereas its nucleotide composition has remained considerably conserved in several taxa. We show that 14 distantly related species within Characoidei share the presence of this satDNA, which is highly amplified and clustered in subtelomeric regions in a single species (Characidiumgomesi), while remained organized as small clusters in all the other species. Defying predictions of the molecular drive of satellite evolution, CharSat01-52 shows similar values of intra- and interspecific divergence. Although we did not provide evidence for a specific functional role of CharSat01-52, its transcriptional activity was demonstrated in different species. In addition, we identified short tandem arrays of CharSat01-52 embedded within single-molecule real-time long reads of Astyanax paranae (536 bp-3.1 kb) and A. mexicanus (501 bp-3.9 kb). Such arrays consisted of head-to-tail repeats and could b e found interspersed with other sequences, inverted sequences, or neighbored by other satellites. Our results provide a detailed characterization of an old and conserved satDNA, challenging general predictions of satDNA evolution.
Subject headings
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
Keyword
- repetitive DNA
- neotropical fish
- tandem repeats
- satDNA
Publication and Content Type
- ref (subject category)
- art (subject category)
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