Search: onr:"swepub:oai:DiVA.org:uu-442723" >
Using Cortical Neur...
Using Cortical Neuron Markers to Target Cells in the Dorsal Cochlear Nucleus
-
- Malfatti, Thawann (author)
- Univ Fed Rio Grande do Norte, Inst Brain, Hearing & Neuronal Act Lab, BR-59056450 Natal, RN, Brazil.
-
- Ciralli, Barbara (author)
- Univ Fed Rio Grande do Norte, Inst Brain, Hearing & Neuronal Act Lab, BR-59056450 Natal, RN, Brazil.
-
- Hilscher, Markus M. (author)
- Vienna Univ Technol, Inst Anal & Sci Comp, A-1040 Vienna, Austria.
-
show more...
-
- Edwards, Steven J. (author)
- Uppsala universitet,Institutionen för neurovetenskap
-
- Kullander, Klas, 1966- (author)
- Uppsala universitet,Funktionella neuronala nätverk
-
- Leao, Richardson N. (author)
- Univ Fed Rio Grande do Norte, Inst Brain, Hearing & Neuronal Act Lab, BR-59056450 Natal, RN, Brazil.
-
- Leao, Katarina E. (author)
- Univ Fed Rio Grande do Norte, Inst Brain, Hearing & Neuronal Act Lab, BR-59056450 Natal, RN, Brazil.
-
show less...
-
Univ Fed Rio Grande do Norte, Inst Brain, Hearing & Neuronal Act Lab, BR-59056450 Natal, RN, Brazil Vienna Univ Technol, Inst Anal & Sci Comp, A-1040 Vienna, Austria. (creator_code:org_t)
- Society for Neuroscience, 2021
- 2021
- English.
-
In: eNeuro. - : Society for Neuroscience. - 2373-2822. ; 8:1
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- The dorsal cochlear nucleus (DCN) is a region of particular interest for auditory and tinnitus research. However, lack of useful genetic markers for in vivo manipulations hinders elucidation of the DCN contribution to tinnitus pathophysiology. This work assesses whether adeno-associated viral vectors (AAV) containing the calcium/calmodulin-dependent protein kinase 2 alpha (CaMKII alpha) promoter and a mouse line of nicotinic acetylcholine receptor alpha 2 subunit (Chrna2)-Cre can target specific DCN populations. We found that CaMKII alpha cannot be used to target excitatory fusiform DCN neurons as labeled cells showed diverse morphology indicating they belong to different classes of DCN neurons. Light stimulation after driving Channelrhodopsin2 (ChR2) by the CaMKIIa promoter generated spikes in some units but firing rate decreased when light stimulation coincided with sound. Expression and activation of CaMKII alpha-eArchaerhodopsin3.0 in the DCN produced inhibition in some units but sound-driven spikes were delayed by concomitant light stimulation. We explored the existence of Cre+ cells in the DCN of Chrna2-Cre mice by hydrogel embedding technique (CLARITY). There were almost no Cre+ cell bodies in the DCN; however, we identified profuse projections arising from the ventral cochlear nucleus (VCN). Anterograde labeling in the VCN revealed projections to the ipsilateral superior olive and contralateral medial nucleus of the trapezoid body (MNTB; bushy cells), and a second bundle terminating in the DCN, suggesting the latter to be excitatory Chrna2+ T-stellate cells. Exciting Chrna2+ cells increased DCN firing. This work shows that cortical molecular tools may be useful for manipulating the DCN especially for tinnitus studies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- CaMKIIa
- Chrna2
- dorsal cochlear nucleus
- extracellular recording
- optogenetics
- ventral cochlear nucleus
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
eNeuro
(Search for host publication in LIBRIS)
To the university's database