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A combined approach...
A combined approach for single-cell mRNA and intracellular protein expression analysis
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- Reimegård, Johan (author)
- Uppsala universitet,Molekylär evolution,Science for Life Laboratory, SciLifeLab,National Bioinformatics Infrastructure Sweden
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- Tarbier, Marcel (author)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Sci Life Lab, Stockholm, Sweden
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- Danielsson, Marcus (author)
- Uppsala universitet,Medicinsk genetik och genomik,Science for Life Laboratory, SciLifeLab
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- Schuster, Jens, Assistant Professor, 1972- (author)
- Uppsala universitet,Medicinsk genetik och genomik
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- Baskaran, Sathishkumar (author)
- Uppsala universitet,Neuroonkologi
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- Panagiotou, Styliani (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Dahl, Niklas (author)
- Uppsala universitet,Medicinsk genetik och genomik
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- Friedländer, Marc R. (author)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Sci Life Lab, Stockholm, Sweden
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- Gallant, Caroline J. (author)
- Uppsala universitet,Molekylära verktyg
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(creator_code:org_t)
- 2021-05-25
- 2021
- English.
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In: Communications Biology. - : Springer Nature. - 2399-3642. ; 4:1
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Abstract
Subject headings
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- Combined measurements of mRNA and protein expression in single cells enable in-depth analysis of cellular states. We present SPARC, an approach that combines single-cell RNA-sequencing with proximity extension essays to simultaneously measure global mRNA and 89 intracellular proteins in individual cells. We show that mRNA expression fails to accurately reflect protein abundance at the time of measurement, although the direction of changes is in agreement during neuronal differentiation. Moreover, protein levels of transcription factors better predict their downstream effects than do their corresponding transcripts. Finally, we highlight that protein expression variation is overall lower than mRNA variation, but relative protein variation does not reflect the mRNA level. Our results demonstrate that mRNA and protein measurements in single cells provide different and complementary information regarding cell states. SPARC presents a state-of-the-art co-profiling method that overcomes current limitations in throughput and protein localization, including removing the need for cell fixation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Reimegård, Johan
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Tarbier, Marcel
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Danielsson, Marc ...
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Schuster, Jens, ...
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Baskaran, Sathis ...
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Panagiotou, Styl ...
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Dahl, Niklas
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Friedländer, Mar ...
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Gallant, Carolin ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Communications B ...
- By the university
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Uppsala University
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Stockholm University