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Diagnostic Potentia...
Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2
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- Hoffman, Tove (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Zoonosis Science Center
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- Kolstad, Linda (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Zoonosis Science Center
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- Lindahl, Johanna (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Zoonosis Science Center
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- Albinsson, Bo (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Uppsala Univ Hosp, Lab Clin Microbiol, Dag Hammarskjolds Vag 38, SE-75237 Uppsala, Sweden.,Zoonosis Science Center
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- Bergqvist, Anders (author)
- Uppsala universitet,Infektionsmedicin,Uppsala Univ Hosp, Lab Clin Microbiol, Dag Hammarskjolds Vag 38, SE-75237 Uppsala, Sweden.
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- Rönnberg, Bengt (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Uppsala Univ Hosp, Lab Clin Microbiol, Dag Hammarskjolds Vag 38, SE-75237 Uppsala, Sweden.,Zoonosis Science Center
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- Lundkvist, Åke (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Zoonosis Science Center
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(creator_code:org_t)
- 2021-05-26
- 2021
- English.
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In: Viruses. - : MDPI. - 1999-4915. ; 13:6
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https://uu.diva-port... (primary) (Raw object)
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Abstract
Subject headings
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- Due to the current, rapidly increasing Coronavirus disease 2019 (COVID-19) pandemic, efficient and highly specific diagnostic methods are needed. The receptor-binding part of the spike (S) protein, S1, has been suggested to be highly virus-specific; it does not cross-react with antibodies against other coronaviruses. Three recombinant partial S proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) expressed in mammalian or baculovirus-insect cells were evaluated as antigens in a Luminex-based suspension immunoassay (SIA). The best performing antigen (S1; amino acids 16-685) was selected and further evaluated by serum samples from 76 Swedish patients or convalescents with COVID-19 (previously PCR and/or serologically confirmed), 200 pre-COVID-19 individuals (180 blood donors and 20 infants), and 10 patients with acute Epstein-Barr virus infection. All 76 positive samples showed detectable antibodies to S1, while none of the 210 negative controls gave a false positive antibody reaction. We further compared the COVID-19 SIA with a commercially available enzyme immunoassay and a previously evaluated COVID-19 rapid antibody test. The results revealed an overall assay sensitivity of 100%, a specificity of 100% for both IgM and IgG, a quantitative ability at concentrations up to 25 BAU/mL, and a better performance as compared to the commercial assays, suggesting the COVID-19 SIA as a most valuable tool for efficient laboratory-based serology.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- COVID-19
- SARS-CoV-2
- Coronavirus
- serology
- IgM
- IgG
Publication and Content Type
- ref (subject category)
- art (subject category)
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