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Stromal Heterogeneity in the Human Proliferative Endometrium-A Single-Cell RNA Sequencing Study
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- Queckboerner, Suzanna (author)
- Karolinska Inst, Div Obstet & Gynecol, Dept Womens & Childrens Hlth, S-17164 Solna, Sweden.;Karolinska Univ Hosp, S-17164 Solna, Sweden.
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- von Grothusen, Carolina (author)
- Karolinska Institutet
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- Boggavarapu, Nageswara Rao (author)
- Karolinska Institutet
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- Karolinska Institutet Karolinska Inst, Div Obstet & Gynecol, Dept Womens & Childrens Hlth, S-17164 Solna, Sweden;Karolinska Univ Hosp, S-17164 Solna, Sweden. (creator_code:org_t)
- 2021-05-22
- 2021
- English.
- In: Journal of Personalized Medicine. - : MDPI. - 2075-4426. ; 11:6
- Journal article (peer-reviewed)
Abstract
Subject headings
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- The endometrium undergoes regular regeneration and stromal proliferation as part of the normal menstrual cycle. To better understand cellular interactions driving the mechanisms in endometrial regeneration we employed single-cell RNA sequencing. Endometrial biopsies were obtained during the proliferative phase of the menstrual cycle from healthy fertile women and processed to single-cell suspensions which were submitted for sequencing. In addition to known endometrial cell types, bioinformatic analysis revealed multiple stromal populations suggestive of specific stromal niches with the ability to control inflammation and extracellular matrix composition. Ten different stromal cells and two pericyte subsets were identified. Applying different R packages (Seurat, SingleR, Velocyto) we established cell cluster diversity and cell lineage/trajectory, while using external data to validate our findings. By understanding healthy regeneration in the described stromal compartments, we aim to identify points of further investigation and possible targets for novel therapy development for benign gynecological disorders affecting endometrial regeneration and proliferation such as endometriosis and Asherman's syndrome.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- endometrium
- endometrial regeneration
- wound healing
- mesenchymal stromal cell
- endometrial pericyte
Publication and Content Type
- ref (subject category)
- art (subject category)
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