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Charge engineering ...
Charge engineering reveals the roles of ionizable side chains in electrospray ionization mass spectrometry
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Abramsson, Mia L (author)
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Sahin, Cagla (author)
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Hopper, Jonathan T S (author)
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Branca, Rui M M (author)
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Danielsson, Jens (author)
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Xu, Mingming (author)
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Chandler, Shane A (author)
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Österlund, Nicklas (author)
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Ilag, Leopold L (author)
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Leppert, Axel (author)
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- Costeira-Paulo, Joana, 1993- (author)
- Uppsala universitet,Biokemi,Marklund group
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Lang, Lisa (author)
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Teilum, Kaare (author)
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Robinson, Carol V (author)
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Laganowsky, Arthur (author)
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Benesch, Justin L P (author)
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Oliveberg, Mikael (author)
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- Marklund, Erik G (author)
- Uppsala universitet,Biokemi,Marklund group
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Allison, Timothy M (author)
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Winther, Jakob R (author)
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Landreh, Michael (author)
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
Close
- The role of ionizable side chains in the electrospray ionization mass spectrometry of intact proteins remains hotly debated but has not been conclusively addressed because multiple chargeable sites are present in virtually all proteins. Using engineered soluble proteins, we show that ionizable side chains are completely dispensable for charging under native conditions, but if present, they are preferential protonation sites. The absence of ionizable side chains results in identical charge state distributions under native-like and denaturing conditions, whilst co-existing conformers can be distinguished using ion mobility separation. An excess of ionizable side chains, on the other hand, effectively modulates protein ion stability. We conclude that the sum of charges is governed solely by Coulombic terms, while their locations affect the stability of the protein in the gas phase.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Native mass spectrometry
- Gas-phase protein structure
- Ionization mechanism
- Protein engineering
- Biokemi
- Biochemistry
Publication and Content Type
- vet (subject category)
- ovr (subject category)
To the university's database
- By the author/editor
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Abramsson, Mia L
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Sahin, Cagla
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Hopper, Jonathan ...
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Branca, Rui M M
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Danielsson, Jens
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Xu, Mingming
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show more...
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Chandler, Shane ...
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Österlund, Nickl ...
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Ilag, Leopold L
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Leppert, Axel
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Costeira-Paulo, ...
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Lang, Lisa
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Teilum, Kaare
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Robinson, Carol ...
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Laganowsky, Arth ...
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Benesch, Justin ...
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Oliveberg, Mikae ...
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Marklund, Erik G
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Allison, Timothy ...
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Winther, Jakob R
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Landreh, Michael
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- By the university
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Uppsala University