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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03528naa a2200373 4500 | |
| 001 | oai:DiVA.org:uu-453474 | |
| 003 | SwePub | |
| 008 | 210923s2021 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4534742 URI |
| 024 | 7 | a https://doi.org/10.1038/s42003-021-02488-12 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Duncan, Kyle D.u Uppsala universitet,Institutionen för kemi - BMC4 aut0 (Swepub:uu)kyldu971 |
| 245 | 1 0 | a In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
| 264 | c 2021-08-13 | |
| 264 | 1 | b Springer Nature,c 2021 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Duncan et al. use a mass spectrometry imaging method to assess the localization and concentration of prostaglandins (PGs) in mouse tissues during pregnancy. This study brings new biological insights into the spatial evaluation of PGs in tissues, which could reveal the functional significance of each PGs during different stages of embryo development/pregnancy. Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localization of regulatory synthases, such as COX1 and COX2. Herein, we apply a novel mass spectrometry imaging method for direct in situ tissue localization of prostaglandins, and combine it with techniques for protein expression and RNA localization. We report that prostaglandin D-2, its precursors, and downstream synthases co-localize with the highest expression of COX1, and not COX2. Further, we study tissue with a conditional deletion of transformation-related protein 53 where pregnancy success is low and confirm that PG levels are altered, although localization is conserved. Our studies reveal that the abundance of COX and prostaglandin D-2 synthases in cellular regions does not mirror the regional abundance of prostaglandins. Thus, we deduce that prostaglandins tissue localization and abundance may not be inferred by COX or prostaglandin synthases in uterine tissue, and must be resolved by an in situ prostaglandin imaging. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 700 | 1 | a Sun, Xiaofeiu Univ Cincinnati, Coll Med, Cincinnati, OH 45221 USA.4 aut |
| 700 | 1 | a Baker, Erin S.u North Carolina State Univ, Dept Chem, Raleigh, NC USA.4 aut |
| 700 | 1 | a Dey, Sudhansu K.u Univ Cincinnati, Coll Med, Cincinnati, OH 45221 USA.4 aut |
| 700 | 1 | a Lanekoff, Ingela,c Assoc. Prof.d 1975-u Uppsala universitet,Analytisk kemi4 aut0 (Swepub:uu)ingla753 |
| 710 | 2 | a Uppsala universitetb Institutionen för kemi - BMC4 org |
| 773 | 0 | t Communications Biologyd : Springer Natureg 4:1q 4:1x 2399-3642 |
| 856 | 4 | u https://doi.org/10.1038/s42003-021-02488-1y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1596716/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://www.nature.com/articles/s42003-021-02488-1.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-453474 |
| 856 | 4 8 | u https://doi.org/10.1038/s42003-021-02488-1 |
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