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Co-occurrence of Fr...
Co-occurrence of Francisella and spotted fever group Rickettsia in avian-associated Hyalomma rufipes
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- Hoffman, Tove (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Sjödin, Andreas (author)
- CBRN Defence and Security, Swedish Defence Research Agency, Cementvägen 20, 90621 Umeå, Sweden
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- Öhrman, Caroline (author)
- CBRN Defence and Security, Swedish Defence Research Agency, Cementvägen 20, 90621 Umeå, Sweden
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- Karlsson, Linda (author)
- CBRN Defence and Security, Swedish Defence Research Agency, Cementvägen 20, 90621 Umeå, Sweden
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- McDonough, Ryelan Francis (author)
- Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA
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- Sahl, Jason W. (author)
- Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA
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- Birdsell, Dawn (author)
- Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA
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- Wagner, David M. (author)
- Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA
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- Carra, Laura G. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Wilhelmsson, Peter (author)
- Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, 581 83 Linköping, Sweden; Department of Clinical Microbiology, Region Jönköping County, 553 05 Jönköping, Sweden
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- Pettersson, John, 1981- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Life and Environmental Sciences and School of Medical Sciences, the University of Sydney, Sydney, New South Wales 2006, Australia
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- Barboutis, Christos (author)
- Antikythira Bird Observatory, Hellenic Ornithological Society/BirdLife Greece, Athens, Greece
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- Figuerola, Jordi (author)
- Estación Biológica de Doñana, CSIC, Avda. Américo Vespucio 26, 410 92 Sevilla, Spain; CIBER Epidemiología y Salud Pública (CIBERESP), 280 29 Madrid, Spain
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- Onrubia, Alejandro (author)
- Migres Foundation, P.O. Box 152, 11380, Tarifa (Cadiz), Spain
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- Kiat, Yosef (author)
- Israeli Bird Ringing Center (IBRC), Israel Ornithological Center (IOC), Society for the Protection of Nature in Israel (SPNI), Tel-Aviv, Israel
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- Piacentini, Dario (author)
- Via Cesare Lippi 35, 400 26, Imola, BO, Italy
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- Jaenson, Thomas G.T. (author)
- Uppsala universitet,Institutionen för organismbiologi
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- Lindgren, Per-Eric (author)
- Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, 581 83 Linköping, Sweden; Department of Clinical Microbiology, Region Jönköping County, 553 05 Jönköping, Sweden
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- Moutailler, Sara (author)
- ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, F-94700, France
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- Fransson, Thord (author)
- Department of Environmental Research and Monitoring, Swedish Museum of Natural History, Stockholm, Sweden
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- Forsman, Mats (author)
- CBRN Defence and Security, Swedish Defence Research Agency, Cementvägen 20, 90621 Umeå, Sweden
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- Nilsson, Kenneth, Docent, 1953- (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Lundkvist, Åke (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Olsen, Björn (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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(creator_code:org_t)
- 2022-07-11
- 2022
- English.
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In: Microorganisms. - : MDPI. - 2076-2607. ; 10:7
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https://doi.org/10.3...
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Abstract
Subject headings
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- Introduction: The migratory behaviour of wild birds aids in the geographical spread of ticks and their microorganisms. Ticks are known to harbor both pathogenic and symbiotic bacteria - such as species of the genera Francisella, Rickettsia,and Midichloria - and multiple bacterial species may occur within them. Francisella occurs in different tick taxa andconsists of closely related pathogenic and non-pathogenic species. Spotted fever group Rickettsia are transmitted to humans by different tick genera and are emerging human pathogens in Europe. The aims of this study were to investigate dispersal of Francisella as well as co-occurrence of Francisella and spotted fever group Rickettsia in ticks infesting northward migrating birds in the African-Western Palaearctic region.Materials and methods: Birds were trapped using mist nets at bird observatories in Spain, Italy, Greece, and Israel during their spring migration of 2014 and 2015. Ticks were screened for the genus Francisella, the species Francisella tularensis, and spotted fever group Rickettsia by microfluidic qPCR. Ticks with putative positive results for F. tularensiswere subjected to confirmation analyses, metagenomics analysis, enrichment, and whole genome sequencing.Results: There was a high prevalence of Francisella species (76.7%) and co-occurrence of Francisella species and spotted fever group Rickettsia (50.6%) in the tick species Hyalomma rufipes. Two H. rufipes yielded putative positive test results for the human pathogen F. tularensis during initial screening. Metagenomics analysis revealed presence of Francisella sp., Rickettsia sp., and Midichloria sp. DNA in the two H. rufipes ticks. The levels of Rickettsia and Midichloria DNA were relatively high while the level of Francisella DNA was low and required enrichment for the construction of metagenome-assembled genomes. Phylogenetic inference and calculations of the average nucleotide identity (ANI) indicated that: i) the Francisella genomes belonged to the Francisella-like endosymbiont (FLE) group in Clade 1 of Francisella and had highest sequence identity to an FLE found in Ornithodoros moubata (ANI: 96.7/97.0%), ii) the Rickettsia genomes had highest resemblance to Rickettsia aeschlimannii (ANI: 98.8 - 99.9%), and iii) the Midichloria genomes resembled Midichloria mitochondrii (ANI: 91.5 - 92.3%).Conclusions: The results of this study suggest ticks containing Francisella species, FLEs, and spotted fever groupRickettsia are dispersed by northbound migratory birds in the African-Western Palaearctic and suggest H. rufipes may not be involved in the transmission of F. tularensis in the study region. Future studies should aim at confirming the prevalence of Francisella spp. and spotted fever group Rickettsia in H. rufipes, in addition to focusing on the influence of FLEs on H. rufipes and their interaction with pathogenic and symbiotic bacteria of the genera Rickettsia and Midichloria.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- African-Western Palaearctic
- migratory birds
- ticks
- Hyalomma rufipes
- Francisella
- Rickettsia
- Midichloria
- PCR
- metagenomics
- Biologi
- Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Hoffman, Tove
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Sjödin, Andreas
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Öhrman, Caroline
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Karlsson, Linda
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McDonough, Ryela ...
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Sahl, Jason W.
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show more...
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Birdsell, Dawn
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Wagner, David M.
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Carra, Laura G.
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Wilhelmsson, Pet ...
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Pettersson, John ...
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Barboutis, Chris ...
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Figuerola, Jordi
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Onrubia, Alejand ...
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Kiat, Yosef
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Piacentini, Dari ...
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Jaenson, Thomas ...
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Lindgren, Per-Er ...
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Moutailler, Sara
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Fransson, Thord
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Forsman, Mats
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Nilsson, Kenneth ...
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Lundkvist, Åke
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Olsen, Björn
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Microorganisms
- By the university
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Uppsala University