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Large scale discove...
Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities
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- Kruse, Thomas (author)
- The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Faculty of Health and Medical Sciences, Blegdamsvej 3B, Copenhagen, Denmark
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- Benz, Caroline (author)
- Uppsala universitet,Biokemi,Department of Chemistry - BMC, Uppsala University, Box 576, Husargatan 3, Uppsala, Sweden
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- Garvanska, Dimitriya H. (author)
- The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Faculty of Health and Medical Sciences, Blegdamsvej 3B, Copenhagen, Denmark
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- Lindquist, Richard, 1985- (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)
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- Mihalic, Filip (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, Husargatan 3, Uppsala, Sweden
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- Coscia, Fabian (author)
- The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Faculty of Health and Medical Sciences, Blegdamsvej 3B, Copenhagen, Denmark; Spatial Proteomics Group, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
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- Inturi, Raviteja, 1985- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, Husargatan 3, Uppsala, Sweden
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- Sayadi, Ahmed (author)
- Uppsala universitet,Biokemi,Department of Chemistry - BMC, Uppsala University, Box 576, Husargatan 3, Uppsala, Sweden
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- Simonetti, Leandro (author)
- Uppsala universitet,Biokemi,Department of Chemistry - BMC, Uppsala University, Box 576, Husargatan 3, Uppsala, Sweden
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- Nilsson, Emma (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)
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- Ali, Muhammad, 1990- (author)
- Karolinska Institutet,Uppsala universitet,Biokemi,Department of Chemistry - BMC, Uppsala University, Box 576, Husargatan 3, Uppsala, Sweden
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- Kliche, Johanna (author)
- Uppsala universitet,Biokemi,Department of Chemistry - BMC, Uppsala University, Box 576, Husargatan 3, Uppsala, Sweden
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- Moliner Morro, Ainhoa (author)
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
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- Mund, Andreas (author)
- The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Faculty of Health and Medical Sciences, Blegdamsvej 3B, Copenhagen, Denmark
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- Andersson, Eva (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, Husargatan 3, Uppsala, Sweden
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- McInerney, Gerald (author)
- Karolinska Institutet,Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
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- Mann, Matthias (author)
- The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Faculty of Health and Medical Sciences, Blegdamsvej 3B, Copenhagen, Denmark
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- Jemth, Per (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, Husargatan 3, Uppsala, Sweden
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- Davey, Norman E. (author)
- Division of Cancer Biology, The Institute of Cancer Research, 237 Fulham Road, London, United Kingdom
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- Överby, Anna K. (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)
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- Nilsson, Jakob (author)
- The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Faculty of Health and Medical Sciences, Blegdamsvej 3B, Copenhagen, Denmark
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- Ivarsson, Ylva (author)
- Uppsala universitet,Biokemi,Department of Chemistry - BMC, Uppsala University, Box 576, Husargatan 3, Uppsala, Sweden
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(creator_code:org_t)
- 2021-11-19
- 2021
- English.
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In: Nature Communications. - : Springer Nature. - 2041-1723. ; 12:1
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Abstract
Subject headings
Close
- Viral proteins make extensive use of short peptide interaction motifs to hijack cellular host factors. However, most current large-scale methods do not identify this important class of protein-protein interactions. Uncovering peptide mediated interactions provides both a molecular understanding of viral interactions with their host and the foundation for developing novel antiviral reagents. Here we describe a viral peptide discovery approach covering 23 coronavirus strains that provides high resolution information on direct virus-host interactions. We identify 269 peptide-based interactions for 18 coronaviruses including a specific interaction between the human G3BP1/2 proteins and an ΦxFG peptide motif in the SARS-CoV-2 nucleocapsid (N) protein. This interaction supports viral replication and through its ΦxFG motif N rewires the G3BP1/2 interactome to disrupt stress granules. A peptide-based inhibitor disrupting the G3BP1/2-N interaction dampened SARS-CoV-2 infection showing that our results can be directly translated into novel specific antiviral reagents.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- General Physics and Astronomy
- General Biochemistry
- Genetics and Molecular Biology
- General Chemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Kruse, Thomas
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Benz, Caroline
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Garvanska, Dimit ...
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Lindquist, Richa ...
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Mihalic, Filip
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Coscia, Fabian
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show more...
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Inturi, Raviteja ...
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Sayadi, Ahmed
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Simonetti, Leand ...
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Nilsson, Emma
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Ali, Muhammad, 1 ...
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Kliche, Johanna
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Moliner Morro, A ...
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Mund, Andreas
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Andersson, Eva
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McInerney, Geral ...
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Mann, Matthias
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Jemth, Per
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Davey, Norman E.
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Överby, Anna K.
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Nilsson, Jakob
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Ivarsson, Ylva
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Nature Communica ...
- By the university
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Uppsala University
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Karolinska Institutet
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Umeå University