Search: onr:"swepub:oai:DiVA.org:uu-461977" >
TGF-beta-mediated e...
-
Miyashita, NaoyaUniv Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.,University of Tokyo
(author)
TGF-beta-mediated epithelial-mesenchymal transition and tumor-promoting effects in CMT64 cells are reflected in the transcriptomic signature of human lung adenocarcinoma
- Article/chapterEnglish2021
Publisher, publication year, extent ...
-
2021-11-17
-
Springer Nature,2021
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-461977
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-461977URI
-
https://doi.org/10.1038/s41598-021-01799-xDOI
-
https://lup.lub.lu.se/record/5277ef74-bd06-452d-82ae-7fdc0a2c59f1URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Epithelial-mesenchymal transition (EMT) is a cellular process during which epithelial cells acquire mesenchymal phenotypes. Cancer cells undergo EMT to acquire malignant features and TGF-beta is a key regulator of EMT. Here, we demonstrate for the first time that TGF-beta could elicit EMT in a mouse lung adenocarcinoma cell line. TGF-beta signaling activation led to cell morphological changes corresponding to EMT and enhanced the expression of mesenchymal markers and EMT-associated transcription factors in CMT64 lung cancer cells. RNA-sequencing analyses revealed that TGF-beta increases expression of Tead transcription factors and an array of Tead2 target genes. TGF-beta stimulation also resulted in alternative splicing of several genes including Cd44, tight junction protein 1 (Tjp1), and Cortactin (Cttn). In parallel with EMT, TGF-beta enhanced cell growth of CMT64 cells and promoted tumor formation in a syngeneic transplantation model. Of clinical importance, the expression of TGF-beta-induced genes identified in CMT64 cells correlated with EMT gene signatures in human lung adenocarcinoma tissue samples. Furthermore, TGF-beta-induced gene enrichment was related to poor prognosis, underscoring the tumor-promoting role of TGF-beta signaling in lung adenocarcinoma. Our cellular and syngeneic transplantation model would provide a simple and useful experimental tool to study the significance of TGF-beta signaling and EMT.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Enokido, TakayoshiUniv Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.,University of Tokyo
(author)
-
Horie, MasafumiOsaka Univ, Grad Sch Med, Dept Canc Genome Informat, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan.,University of Tokyo
(author)
-
Fukuda, KensukeUniv Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.,University of Tokyo
(author)
-
Urushiyama, HirokazuUniv Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.
(author)
-
Strell, CarinaUppsala University,Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke(Swepub:uu)carst810
(author)
-
Brunnström, HansLund University,Lunds universitet,Förbättrad diagnostik och prognostik vid lungcancer och metastaser till lunga,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Patologi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Improved diagnostics and prognostics of lung cancer and metastases to the lungs,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Pathology, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)med-hsb
(author)
-
Micke, PatrickUppsala University,Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke(Swepub:uu)patmi676
(author)
-
Saito, AkiraUniv Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.,University of Tokyo
(author)
-
Nagase, TakahideUniv Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.,University of Tokyo
(author)
-
Univ Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.University of Tokyo
(creator_code:org_t)
Related titles
-
In:Scientific Reports: Springer Nature112045-2322
Internet link
Find in a library
To the university's database
- By the author/editor
-
Miyashita, Naoya
-
Enokido, Takayos ...
-
Horie, Masafumi
-
Fukuda, Kensuke
-
Urushiyama, Hiro ...
-
Strell, Carina
-
show more...
-
Brunnström, Hans
-
Micke, Patrick
-
Saito, Akira
-
Nagase, Takahide
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Clinical Laborat ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cancer and Oncol ...
- Articles in the publication
-
Scientific Repor ...
- By the university
-
Uppsala University
-
Lund University