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Conditions for main...
Conditions for maintenance of hepatocyte differentiation and function in 3D cultures
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- Handin, Niklas (author)
- Uppsala universitet,Institutionen för farmaci
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- Mickols, Evgeniya (author)
- Uppsala universitet,Institutionen för farmaci
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- Ölander, Magnus (author)
- Uppsala universitet,Institutionen för farmaci
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- Rudfeldt, Jakob (author)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin
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- Blom, Kristin (author)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin,Klinisk kemi
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- Nyberg, Frida (author)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin
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- Senkowski, Wojciech (author)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin,Uppsala Univ, Dept Med Sci, Div Canc Pharmacol & Computat Med, Uppsala, Sweden.;Univ Copenhagen, Biotech Res & Innovat Ctr BRIC, DK-2200 Copenhagen N, Denmark.;Univ Copenhagen, Novo Nordisk Fdn Ctr, Stem Cell Biol DanStem, DK-2200 Copenhagen N, Denmark.
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- Urdzik, Jozef (author)
- Uppsala universitet,Gastrointestinalkirurgi
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- Maturi, Varun (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Institutionen för farmaci
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- Fryknäs, Mårten (author)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin
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- Artursson, Per (author)
- Uppsala universitet,Institutionen för farmaci,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- Cell Press, 2021
- 2021
- English.
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In: iScience. - : Cell Press. - 2589-0042. ; 24:11
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Abstract
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- Spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. The cultures are maintained under different cone-lions, with possible confounding results. We performed an in-depth analysis of the influence of various culture conditions to find the optimal conditions for the maintenance of an in vivo like phenotype. The formation, protein expression, and function of PHH spheroids were followed for three weeks in a high-throughput 384-well format. Medium composition affected spheroid histology, global proteome profile, drug metabolism and drug-induced toxicity. No epithelial-mesenchymel transition was observed. Media with fasting glucose and insulin levels gave spheroids with phenotypes closest to normal PHH. The most expensive medium resulted in PHH features most divergent from that of native PHH. Our results provide a protocol for culture of healthy PHH with maintained function a prerequisite for studies of hepatocyte homeostasis and more reproducible hepatocyte research.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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iScience
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To the university's database
- By the author/editor
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Handin, Niklas
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Mickols, Evgeniy ...
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Ölander, Magnus
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Rudfeldt, Jakob
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Blom, Kristin
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Nyberg, Frida
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show more...
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Senkowski, Wojci ...
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Urdzik, Jozef
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Maturi, Varun
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Fryknäs, Mårten
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Artursson, Per
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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iScience
- By the university
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Uppsala University