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Targeting hepatocyt...
Targeting hepatocyte growth factor in epithelial-stromal interactions in an in vitro experimental model of human periodontitis
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Yamaguchi, Yoko (author)
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Saito, Akira (author)
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Horie, Masafumi (author)
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Aoki, Akira (author)
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- Micke, Patrick (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Patrick Micke
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Ohshima, Mitsuhiro (author)
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Kappert, Kai (author)
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(creator_code:org_t)
- 2021-06-14
- 2021
- English.
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In: Odontology. - : Springer Nature. - 1618-1247 .- 1618-1255. ; 109:4, s. 912-920
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Abstract
Subject headings
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- Periodontitis is a chronic inflammatory disease leading to progressive connective tissue degradation and loss of the tooth-supporting bone. Clinical and experimental studies suggest that hepatocyte growth factor (HGF) is involved in the dysregulated fibroblast-epithelial cell interactions in periodontitis. The aim of this study was to explore effects of HGF to impact fibroblast-induced collagen degradation. A patient-derived experimental cell culture model of periodontitis was applied. Primary human epithelial cells and fibroblasts isolated from periodontitis-affected gingiva were co-cultured in a three-dimensional collagen gel. The effects of HGF neutralizing antibody on collagen gel degradation were tested and transcriptome analyses were performed. HGF neutralizing antibody attenuated collagen degradation and elicited expression changes of genes related to extracellular matrix (ECM) and cell adhesion, indicating that HGF signaling inhibition leads to extensive impact on cell-cell and cell-ECM interactions. Our study highlights a potential role of HGF in periodontitis. Antagonizing HGF signaling by a neutralizing antibody may represent a novel approach for periodontitis treatment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Keyword
- Hepatocyte growth factor
- Periodontitis
- Human primary cell
- Extracellular matrix
- Transcriptome
- Pathology
- Patologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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