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TIDE Analysis of Cryptosporidium Infections by gp60 Typing Reveals Obscured Mixed Infections

Dettwiler, Ines (author)
Univ Bern, Inst Anim Pathol, Laenggassstr 122, CH-3012 Bern, Switzerland.
Troell, Karin (author)
Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Natl Vet Inst, Dept Microbiol, Uppsala, Sweden.
Robinson, Guy (author)
Singleton Hosp, Cryptosporidium Reference Unit, Publ Hlth Wales Microbiol, Swansea, W Glam, Wales.;Swansea Univ, Med Sch, Swansea, W Glam, Wales.
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Chalmers, Rachel M. (author)
Singleton Hosp, Cryptosporidium Reference Unit, Publ Hlth Wales Microbiol, Swansea, W Glam, Wales.;Swansea Univ, Med Sch, Swansea, W Glam, Wales.
Basso, Walter (author)
Univ Bern, Inst Parasitol, Bern, Switzerland.
Renteria-Solis, Zaida Melina (author)
Univ Leipzig, Inst Parasitol, Leipzig, Germany.
Daugschies, Arwid (author)
Univ Leipzig, Inst Parasitol, Leipzig, Germany.
Muhlethaler, Konrad (author)
Univ Bern, Inst Infect Dis, Bern, Switzerland.
Dale, Mariko, I (author)
Univ Bern, Inst Anim Pathol, Laenggassstr 122, CH-3012 Bern, Switzerland.
Raghavendra, Jyothi Basapathi (author)
Univ Bern, Inst Anim Pathol, Laenggassstr 122, CH-3012 Bern, Switzerland.
Ruf, Marie-Therese (author)
Swiss Trop & Publ Hlth Inst, Basel, Switzerland.;Univ Basel, Basel, Switzerland.
Poppert, Sven (author)
Swiss Trop & Publ Hlth Inst, Basel, Switzerland.;Univ Basel, Basel, Switzerland.
Meylan, Mireille (author)
Univ Bern, Clin Ruminants, Bern, Switzerland.
Olias, Philipp (author)
Univ Bern, Inst Anim Pathol, Laenggassstr 122, CH-3012 Bern, Switzerland.
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Univ Bern, Inst Anim Pathol, Laenggassstr 122, CH-3012 Bern, Switzerland Institutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)
2021-08-21
2022
English.
In: Journal of Infectious Diseases. - : Oxford University Press (OUP). - 0022-1899 .- 1537-6613. ; 225:4, s. 686-695
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Background. Cryptosporidiosis is a parasitic disease associated with potentially fatal diarrhea. The most used method in Cryptosporidium subtyping is based on the glycoprotein gene gp60. Each infection can represent a parasite population, and it is important to investigate the influence on transmission and virulence, as well as any impact on public health investigations. However, an easy-to-use method for detection is lacking. Methods. Here we report on the use of the bioinformatic program TIDE for deconvolution of gp60 chromatograms. A combination of single oocyst analysis and cloning successfully confirmed the within-sample parasite population diversity. Retrospective sample analysis was conducted on archived chromatograms. Results. For Cryptosporidium parvum, 8.6% multistrain infections (13 of 152) obscured by currently used consensus base calling were detected. Importantly, we show that single oocysts can harbor a mixed population of sporozoites. We also identified a striking dominance of unappreciated polymerase stutter artefacts in all 218 chromatograms analyzed, challenging the uncritical use of gp60 typing. Conclusions. We demonstrate the value of a new, easy-to-use analytical procedure for critical characterization of C. parvum and Cryptosporidium hominis in epidemiological investigations, also applicable retrospectively. Our findings illuminate the hidden parasite diversity with important implications for tracing zoonotic and person-to-person transmissions.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Keyword

Apicomplexa
forensics
protozoa
stutter
zoonosis

Publication and Content Type

ref (subject category)
art (subject category)

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