Use of metagenomic microbial source tracking to investigate the source of a foodborne outbreak of cryptosporidiosis

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:uu-472395" > Use of metagenomic ...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Ahlinder, J.FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden. (author)

Use of metagenomic microbial source tracking to investigate the source of a foodborne outbreak of cryptosporidiosis

Article/chapterEnglish2022

Publisher, publication year, extent ...

Elsevier,2022
electronicrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:uu-472395
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-472395URI
https://doi.org/10.1016/j.fawpar.2021.e00142DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Cryptosporidium is a protozoan parasite of global public health importance that causes gastroenteritis in a variety of vertebrate hosts, with many human outbreaks reported yearly, often from ingestion of contaminated water or food. Despite the major public health implications, little is typically known about sources of contamination of disease outbreaks caused by Cryptosporidium. Here, we study a national foodborne outbreak resulted from infection with Cryptosporidium parvum via romaine lettuce, with the main goal to trace the source of the parasite. To do so, we combined traditional outbreak investigation methods with molecular detection and characterization methods (i.e. PCR based typing, amplicon and shotgun sequencing) of romaine lettuce samples collected at the same farm from which the contaminated food was produced. Using 18S rRNA typing, we detected C. parvum in two out of three lettuce samples, which was supported by detections in the metagenome analysis. Microbial source tracking analysis of the lettuce samples suggested sewage water as a likely source of the contamination, albeit with some uncertainty. In addition, the high degree of overlap in bacterial species content with a public human gut microbial database corroborated the source tracking results. The combination of traditional and molecular based methods applied here is a promising tool for future source tracking investigations of food- and waterborne outbreaks of Cryptosporidium spp. and can help to control and mitigate contamination risks.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Infektionsmedicin hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Infectious Medicine hsv//eng
Vegetables
Contaminated food
Cryptosporidium parvum
Foodborne outbreak investigation
18S rRNA
16S rRNA

Added entries (persons, corporate bodies, meetings, titles ...)

Svedberg, A-LReg Norrbotten, Dept Infect Control, Luleå, Sweden. (author)
Nystedt, A.Reg Norrbotten, Dept Infect Control, Luleå, Sweden. (author)
Dryselius, R.Natl Food Agcy, Dept Biol, Uppsala, Sweden. (author)
Jacobsson, K.Natl Food Agcy, Dept Biol, Uppsala, Sweden. (author)
Hägglund, M.Clin Genom Facil, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden. (author)
Brindefalk, B.FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden. (author)
Forsman, M.FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden. (author)
Ottoson, J.Natl Food Agcy, Dept Risk & Benefit Assessment, Uppsala, Sweden. (author)
Troell, KarinUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Natl Vet Inst, Dept Microbiol, Uppsala, Sweden.(Swepub:uu)kartr688 (author)
FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.Reg Norrbotten, Dept Infect Control, Luleå, Sweden. (creator_code:org_t)

Related titles

In:FOOD AND WATERBORNE PARASITOLOGY: Elsevier262405-6766

Internet link

Find in a library

FOOD AND WATERBORNE PARASITOLOGY (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Ahlinder, J.; Svedberg, A-L; Nystedt, A.; Dryselius, R.; Jacobsson, K.; Hägglund, M.; show more...; Brindefalk, B.; Forsman, M.; Ottoson, J.; Troell, Karin; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Infectious Medic ...

Articles in the publication: FOOD AND WATERBO ...

By the university: Uppsala University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se