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SLC38A10 deficiency...
SLC38A10 deficiency in male mice affect plasma levels of threonine and histidine
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- Lindberg, Frida A (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Molecular Neuropharmacology
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- Nordenankar, Karin (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Molecular Neuropharmacology
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- Forsberg, Erica C (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Molecular Neuropharmacology
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- Fredriksson, Robert (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Molecular Neuropharmacology
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(creator_code:org_t)
- English.
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https://urn.kb.se/re...
Abstract
Subject headings
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- Solute carriers belong to the biggest group of transporters in the human genome, but more knowledge is needed in order to fully understand their function and possible role as therapeutic targets. SLC38 is a family of amino acid transporters, commonly referred to as SNATs, consisting of 11 members. The tenth member, SLC38A10, is one of the least characterized members and is the focus of this study. By using a knockout mouse model, we studied the biological effects of SLC38A10 deficiency in vivo. We performed a transcriptomic analysis of whole brain and found seven differentially expressed genes in SLC38A10 deficient mice (Gm48159, Nr4a1, Tuba1c, Lrrc56, mt-Tp, Hbb-bt and Snord116/9). By measuring amino acids in plasma, we found lower levels of threonine and histidine in males, while no amino acids were altered in females, suggesting that SLC38A10-/- might affect sexes differently. Using RT-qPCR, we investigated the effect of SLC38A10 deficiency on mRNA expression of other SLC38 members, Mtor and Rps6kb1 in brain, liver, lung, muscle and kidney, but no differences were found. A relative telomere length measurement was also made, as a marker for cellular age, but no differences were found between the genotypes. We conclude that SLC38A10 might be important for keeping amino acid homeostasis in plasma, at least in males, but no major effects were seen on transcriptomic expression or telomere length in whole brain.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Slc38a10
- SNAT10
- knockout
- amino acid homeostasis
- gene expression analysis
Publication and Content Type
- vet (subject category)
- ovr (subject category)
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