Search: onr:"swepub:oai:DiVA.org:uu-473728" >
Functional, metabol...
Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells.
-
Balboa, Diego (author)
-
Barsby, Tom (author)
-
Lithovius, Väinö (author)
-
show more...
-
Saarimäki-Vire, Jonna (author)
-
- Omar-Hmeadi, Muhmmad (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Dyachok, Oleg, 1965- (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
Montaser, Hossam (author)
-
- Lund, Per-Eric (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Yang, Mingyu (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
Ibrahim, Hazem (author)
-
Näätänen, Anna (author)
-
Chandra, Vikash (author)
-
Vihinen, Helena (author)
-
Jokitalo, Eija (author)
-
Kvist, Jouni (author)
-
Ustinov, Jarkko (author)
-
Nieminen, Anni I (author)
-
Kuuluvainen, Emilia (author)
-
Hietakangas, Ville (author)
-
- Katajisto, Pekka (author)
- Karolinska Institutet
-
- Lau, Joey, 1979- (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Carlsson, Per-Ola (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Barg, Sebastian, 1969- (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Tengholm, Anders, 1971- (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
Otonkoski, Timo (author)
-
show less...
-
(creator_code:org_t)
- 2022-03-03
- 2022
- English.
-
In: Nature Biotechnology. - : Springer Nature. - 1087-0156 .- 1546-1696. ; 40:7, s. 1042-1055
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://www.nature.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Transplantation of pancreatic islet cells derived from human pluripotent stem cells is a promising treatment for diabetes. Despite progress in the generation of stem-cell-derived islets (SC-islets), no detailed characterization of their functional properties has been conducted. Here, we generated functionally mature SC-islets using an optimized protocol and benchmarked them comprehensively against primary adult islets. Biphasic glucose-stimulated insulin secretion developed during in vitro maturation, associated with cytoarchitectural reorganization and the increasing presence of alpha cells. Electrophysiology, signaling and exocytosis of SC-islets were similar to those of adult islets. Glucose-responsive insulin secretion was achieved despite differences in glycolytic and mitochondrial glucose metabolism. Single-cell transcriptomics of SC-islets in vitro and throughout 6 months of engraftment in mice revealed a continuous maturation trajectory culminating in a transcriptional landscape closely resembling that of primary islets. Our thorough evaluation of SC-islet maturation highlights their advanced degree of functionality and supports their use in further efforts to understand and combat diabetes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Balboa, Diego
-
Barsby, Tom
-
Lithovius, Väinö
-
Saarimäki-Vire, ...
-
Omar-Hmeadi, Muh ...
-
Dyachok, Oleg, 1 ...
-
show more...
-
Montaser, Hossam
-
Lund, Per-Eric
-
Yang, Mingyu
-
Ibrahim, Hazem
-
Näätänen, Anna
-
Chandra, Vikash
-
Vihinen, Helena
-
Jokitalo, Eija
-
Kvist, Jouni
-
Ustinov, Jarkko
-
Nieminen, Anni I
-
Kuuluvainen, Emi ...
-
Hietakangas, Vil ...
-
Katajisto, Pekka
-
Lau, Joey, 1979-
-
Carlsson, Per-Ol ...
-
Barg, Sebastian, ...
-
Tengholm, Anders ...
-
Otonkoski, Timo
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Endocrinology an ...
- Articles in the publication
-
Nature Biotechno ...
- By the university
-
Uppsala University
-
Karolinska Institutet