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- Balboa, Diego (author)
Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells.
- Article/chapterEnglish2022
Publisher, publication year, extent ...
- 2022-03-03
- Springer Nature,2022
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-473728
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-473728URI
- https://doi.org/10.1038/s41587-022-01219-zDOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:149272207URI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Transplantation of pancreatic islet cells derived from human pluripotent stem cells is a promising treatment for diabetes. Despite progress in the generation of stem-cell-derived islets (SC-islets), no detailed characterization of their functional properties has been conducted. Here, we generated functionally mature SC-islets using an optimized protocol and benchmarked them comprehensively against primary adult islets. Biphasic glucose-stimulated insulin secretion developed during in vitro maturation, associated with cytoarchitectural reorganization and the increasing presence of alpha cells. Electrophysiology, signaling and exocytosis of SC-islets were similar to those of adult islets. Glucose-responsive insulin secretion was achieved despite differences in glycolytic and mitochondrial glucose metabolism. Single-cell transcriptomics of SC-islets in vitro and throughout 6 months of engraftment in mice revealed a continuous maturation trajectory culminating in a transcriptional landscape closely resembling that of primary islets. Our thorough evaluation of SC-islet maturation highlights their advanced degree of functionality and supports their use in further efforts to understand and combat diabetes.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
Added entries (persons, corporate bodies, meetings, titles ...)
- Barsby, Tom (author)
- Lithovius, Väinö (author)
- Saarimäki-Vire, Jonna (author)
- Omar-Hmeadi, MuhmmadUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)muhhm810 (author)
- Dyachok, Oleg,1965-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)olegdyac (author)
- Montaser, Hossam (author)
- Lund, Per-EricUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)peerlund (author)
- Yang, MingyuUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)minya266 (author)
- Ibrahim, Hazem (author)
- Näätänen, Anna (author)
- Chandra, Vikash (author)
- Vihinen, Helena (author)
- Jokitalo, Eija (author)
- Kvist, Jouni (author)
- Ustinov, Jarkko (author)
- Nieminen, Anni I (author)
- Kuuluvainen, Emilia (author)
- Hietakangas, Ville (author)
- Katajisto, PekkaKarolinska Institutet (author)
- Lau, Joey,1979-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)jolau226 (author)
- Carlsson, Per-OlaUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)perocarl (author)
- Barg, Sebastian,1969-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)sebba507 (author)
- Tengholm, Anders,1971-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)andeteng (author)
- Otonkoski, Timo (author)
- Uppsala universitetInstitutionen för medicinsk cellbiologi (creator_code:org_t)
Related titles
- In:Nature Biotechnology: Springer Nature40:7, s. 1042-10551087-01561546-1696
Internet link
- https://doi.org/10.1038/s41587-022-01219-zFulltext
- https://uu.diva-portal.org/smash/get/diva2:1655667/FULLTEXT01.pdffulltext:print
- https://www.nature.com/articles/s41587-022-01219-z.pdf
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-473728
- https://doi.org/10.1038/s41587-022-01219-z
- http://kipublications.ki.se/Default.aspx?queryparsed=id:149272207
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