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Observations of pha...
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Wells, Daniel J.La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia.
(författare)
Observations of phase changes in monoolein during high viscous injection
- Artikel/kapitelEngelska2022
Förlag, utgivningsår, omfång ...
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International Union Of Crystallography,2022
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-476108
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-476108URI
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https://doi.org/10.1107/S1600577522001862DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-313313URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20220607
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Serial crystallography of membrane proteins often employs high-viscosity injectors (HVIs) to deliver micrometre-sized crystals to the X-ray beam. Typically, the carrier medium is a lipidic cubic phase (LCP) media, which can also be used to nucleate and grow the crystals. However, despite the fact that the LCP is widely used with HVIs, the potential impact of the injection process on the LCP structure has not been reported and hence is not yet well understood. The self-assembled structure of the LCP can be affected by pressure, dehydration and temperature changes, all of which occur during continuous flow injection. These changes to the LCP structure may in turn impact the results of X-ray diffraction measurements from membrane protein crystals. To investigate the influence of HVIs on the structure of the LCP we conducted a study of the phase changes in monoolein/water and monoolein/buffer mixtures during continuous flow injection, at both atmospheric pressure and under vacuum. The reservoir pressure in the HVI was tracked to determine if there is any correlation with the phase behaviour of the LCP. The results indicated that, even though the reservoir pressure underwent (at times) significant variation, this did not appear to correlate with observed phase changes in the sample stream or correspond to shifts in the LCP lattice parameter. During vacuum injection, there was a three-way coexistence of the gyroid cubic phase, diamond cubic phase and lamellar phase. During injection at atmospheric pressure, the coexistence of a cubic phase and lamellar phase in the monoolein/water mixtures was also observed. The degree to which the lamellar phase is formed was found to be strongly dependent on the co-flowing gas conditions used to stabilize the LCP stream. A combination of laboratory-based optical polarization microscopy and simulation studies was used to investigate these observations.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Berntsen, PeterLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Balaur, EugeniuLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Kewish, Cameron M.Australian Nucl Sci & Technol Org, Australian Synchrotron, 800 Blackburn Rd, Clayton, Vic 3168, Australia.;La Trobe Univ, Sch Mol Sci, Dept Chem & Phys, Bundoora, Vic 3086, Australia
(författare)
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Adams, PatrickRMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
(författare)
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Sellberg, Jonas A.,1985-KTH,Biomedicinsk fysik och röntgenfysik,SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden(Swepub:kth)u1ynvdbz
(författare)
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Binns, JackRMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
(författare)
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Boutet, SebastienSLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
(författare)
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Broomhall, HaydenRMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
(författare)
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Caleman, CarlUppsala universitet,Institutionen för fysik och astronomi,DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany(Swepub:uu)cajca839
(författare)
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Christofferson, AndrewRMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
(författare)
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Conn, Charlotte E.RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
(författare)
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Dahlqvist, CarolineKTH,AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden.,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden
(författare)
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Flueckiger, LeonieLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Roque, Francisco GianLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Greaves, Tamar L.RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
(författare)
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Hejazian, MajidLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Hunter, MarkSLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
(författare)
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Jazi, Marjan HadianLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Jönsson, H. OlofKTH,AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden.,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden
(författare)
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Pathirannahalage, Sachini KadaoluwaRMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
(författare)
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Kirian, Richard A.Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA,La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia.
(författare)
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Kozlov, AlexUniv Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
(författare)
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Kurta, Ruslan P.European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany
(författare)
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Marman, HughLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Mendez, DerekArizona State Univ, Dept Phys, Tempe, AZ 85287 USA
(författare)
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Morgan, AndrewUniv Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
(författare)
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Nugent, KeithAustralian Natl Univ, Res Sch Phys, Acton, ACT, Australia
(författare)
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Oberthuer, DominikDESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany
(författare)
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Quiney, HarryUniv Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
(författare)
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Reinhardt, JulianeAustralian Nucl Sci & Technol Org, Australian Synchrotron, 800 Blackburn Rd, Clayton, Vic 3168, Australia
(författare)
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Saha, SaumitraUniv Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
(författare)
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Sellberg, Jonas A.KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden
(författare)
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Sierra, RaymondSLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
(författare)
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Wiedorn, MaxDESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany
(författare)
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Abbey, BrianLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(författare)
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Martin, Andrew, VRMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia.
(författare)
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Darmanin, ConnieLa Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia.
(författare)
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La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia.La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Synchrotron Radiation: International Union Of Crystallography29:3, s. 602-6140909-04951600-5775
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Till lärosätets databas
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Wells, Daniel J.
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Berntsen, Peter
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Balaur, Eugeniu
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Kewish, Cameron ...
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Adams, Patrick
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Sellberg, Jonas ...
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visa fler...
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Binns, Jack
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Boutet, Sebastie ...
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Broomhall, Hayde ...
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Caleman, Carl
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Christofferson, ...
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Conn, Charlotte ...
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Dahlqvist, Carol ...
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Flueckiger, Leon ...
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Roque, Francisco ...
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Greaves, Tamar L ...
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Hejazian, Majid
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Hunter, Mark
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Jazi, Marjan Had ...
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Jönsson, H. Olof
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Pathirannahalage ...
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Kirian, Richard ...
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Kozlov, Alex
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Kurta, Ruslan P.
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Marman, Hugh
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Mendez, Derek
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Morgan, Andrew
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Nugent, Keith
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Oberthuer, Domin ...
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Quiney, Harry
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Reinhardt, Julia ...
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Saha, Saumitra
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Sellberg, Jonas ...
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Sierra, Raymond
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Wiedorn, Max
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Abbey, Brian
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Martin, Andrew, ...
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Darmanin, Connie
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