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The effects of xeno...
The effects of xeno-free cryopreservation on the contractile properties of human iPSC derived cardiomyocytes
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- Chirikian, Orlando (author)
- UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA.;UC Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA USA.;Stanford Univ, Stanford, CA USA.
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- Feinstein, Samuel D. (author)
- UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA.
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- Faynus, Mohamed A. (author)
- UC Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA USA.
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- Kim, Anna A (author)
- Uppsala universitet,Institutionen för materialvetenskap,UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA.
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- Lane, Kerry V. (author)
- UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA.
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- Torres, Gabriela V. (author)
- UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA.
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- Pham, Jeffrey V. (author)
- UC Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA.
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- Singh, Zachary (author)
- UC Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA.
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- Nguyen, Amanda (author)
- UC Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA.
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- Thomas, Dilip (author)
- Stanford Univ, Stanford, CA USA.;Stanford Cardiovasc Inst, Stanford, CA USA.
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- Clegg, Dennis O. (author)
- UC Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA.;UC Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA USA.
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- Wu, Joseph C. (author)
- Stanford Univ, Stanford, CA USA.;Stanford Cardiovasc Inst, Stanford, CA USA.;Dept Med, Div Cardiovasc Med, Stanford, CA USA.;Inst Stem Cell Biol & Regenerat Med, Stanford, CA USA.
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- Pruitt, Beth L. (author)
- UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA.;UC Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA USA.;UC Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA USA.;Univ Calif Santa Barbara, Dept Mech Engn, 2002 Bioengn Bldg, Santa Barbara, CA 93106 USA.
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UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA;UC Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA USA.;Stanford Univ, Stanford, CA USA. UC Santa Barbara, Dept Mech Engn, Santa Barbara, CA USA. (creator_code:org_t)
- Elsevier, 2022
- 2022
- English.
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In: Journal of Molecular and Cellular Cardiology. - : Elsevier. - 0022-2828 .- 1095-8584. ; 168, s. 107-114
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Abstract
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- Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have advanced our ability to study the basic function of the heart and model cardiac diseases. Due to the complexities in stem cell culture and differentiation protocols, many researchers source their hiPSC-CMs from collaborators or commercial biobanks. Generally, the field has assumed the health of frozen cardiomyocytes is unchanged if the cells adhere to the substrate and commence beating. However, very few have investigated the effects of cryopreservation on hiPSCCM's functional and transcriptional health at the cellular and molecular level. Here we review methods and challenges associated with cryopreservation, and examine the effects of cryopreservation on the functionality (contractility and calcium handling) and transcriptome of hiPSC-CMs from six healthy stem cell lines. Utilizing protein patterning methods to template physiological cell aspect ratios (7:1, length:width) in conjunction with polyacrylamide (PA) hydrogels, we measured changes in force generation and calcium handling of single hiPSCCMs. We observed that cryopreservation altered the functionality and transcriptome of hiPSC-CMs towards larger sizes and contractile force as assessed by increased spread area and volume, single cell traction force microscopy and delayed calcium dynamics. hiPSC-CMs are broadly used for basic science research, regenerative medicine, and testing biological therapeutics. This study informs the design of experiments utilizing hiPSC-CMs to avoid confounding functional changes due to cryopreservation with other treatments.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Human induced pluripotent cells
- Cardiomyocytes
- Cryopreservation
- Viability
- Functionality
Publication and Content Type
- ref (subject category)
- art (subject category)
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Chirikian, Orlan ...
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Feinstein, Samue ...
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Faynus, Mohamed ...
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Kim, Anna A
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Lane, Kerry V.
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Torres, Gabriela ...
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show more...
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Pham, Jeffrey V.
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Singh, Zachary
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Nguyen, Amanda
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Thomas, Dilip
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Clegg, Dennis O.
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Wu, Joseph C.
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Pruitt, Beth L.
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Uppsala University