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Microfluidics platf...
Microfluidics platform for studies of peptide - polyelectrolyte interaction
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- Wanselius, Marcus (author)
- Uppsala universitet,Institutionen för läkemedelskemi
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- Searle, Sean, 1991- (author)
- Uppsala universitet,Institutionen för läkemedelskemi
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- Rodler, Agnes (author)
- Uppsala universitet,Institutionen för läkemedelskemi
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- Tenje, Maria (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för materialvetenskap,EMBLA
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- Abrahmsén-Alami, Susanna (author)
- AstraZeneca, Innovat Strategies & External Liaison Pharmaceut T, Operat, Gothenburg, Sweden.
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- Hansson, Per (author)
- Uppsala universitet,Institutionen för läkemedelskemi
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(creator_code:org_t)
- Elsevier, 2022
- 2022
- English.
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In: International Journal of Pharmaceutics. - : Elsevier. - 0378-5173 .- 1873-3476. ; 621
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Abstract
Subject headings
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- Subcutaneous injection is one of the most common approaches for administering biopharmaceuticals unsuitable for oral delivery. However, there is a lack of methods to predict the behavior of biopharmaceuticals within the extracellular matrix of the subcutaneous tissue. In this work, we present a novel miniaturized microfluidic-based in vitro method able to investigate interactions between drug molecules and the polymers of the subcutaneous extracellular matrix. To validate the method, microgels consisting of, respectively, covalently cross-linked hy-aluronic acid, polyacrylic acid, and commercially available DC BeadTM, were exposed to three model substances: cytochrome C, protamine sulfate and amitriptyline hydrochloride. These components were chosen to include systems with widely different physiochemical properties (charge, size, self-assembly, etc.) The experimental results were compared with theoretical predictions from a gel model developed earlier. The results show that the method is suitable as a rapid screening method for automated, large-scale, probing of interactions between biopolymers and drug molecules, with small consumption of material.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Keyword
- Microfluidics
- Microgel
- Subcutaneous administration
- In vitro method
- Polyelectrolyte
- Peptide
- Protein
- Drug
Publication and Content Type
- ref (subject category)
- art (subject category)
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