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Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II
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- Han, Guangye (author)
- Uppsala universitet,Molekylär biomimetik,Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China.,Molecular Biomimetics, Department of Chemistry, Ångström Laboratory, Uppsala University, Box 523, Uppsala, Sweden
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- Chernev, Petko (author)
- Uppsala universitet,Molekylär biomimetik,Molecular Biomimetics, Department of Chemistry, Ångström Laboratory, Uppsala University, Box 523, Uppsala, Sweden
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- Styring, Stenbjörn (author)
- Uppsala universitet,Molekylär biomimetik,Molecular Biomimetics, Department of Chemistry, Ångström Laboratory, Uppsala University, Box 523, Uppsala, Sweden
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- (creator_code:org_t)
- 2022
- 2022
- English.
- In: Chemical Science. - : Royal Society of Chemistry. - 2041-6520 .- 2041-6539. ; 13:29, s. 8667-8678
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Photosynthesis stores solar light as chemical energy and efficiency of this process isv highly important. The electrons required for CO2 reduction are extracted from water in a reaction driven by light-induced charge separations in the Photosystem II reaction center and catalyzed by the CaMn4O5-cluster. This cyclic process involves five redox intermediates known as the S-0-S-4 states. In this study, we quantify the flash-induced turnover efficiency of each S state by electron paramagnetic resonance spectroscopy. Measurements were performed in photosystem II membrane preparations from spinach in the presence of an exogenous electron acceptor at selected temperatures between -10 degrees C and +20 degrees C and at flash frequencies of 1.25, 5 and 10 Hz. The results show that at optimal conditions the turnover efficiencies are limited by reactions occurring in the water oxidizing complex, allowing the extraction of their S state dependence and correlating low efficiencies to structural changes and chemical events during the reaction cycle. At temperatures 10 degrees C and below, the highest efficiency (i.e. lowest miss parameter) was found for the S-1 -> S-2 transition, while the S-2 -> S-3 transition was least efficient (highest miss parameter) over the whole temperature range. These electron paramagnetic resonance results were confirmed by measurements of flash-induced oxygen release patterns in thylakoid membranes and are explained on the basis of S state dependent structural changes at the CaMn4O5-cluster that were determined recently by femtosecond X-ray crystallography. Thereby, possible "molecular errors" connected to the e(-) transfer, H+ transfer, H2O binding and O-2 release are identified.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
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