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Independently paced...
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Kim, Anna A.Uppsala universitet,Institutionen för materialvetenskap,Stanford University School of Medicine; University of California
(författare)
Independently paced Ca2+ oscillations in progenitor and differentiated cells in an ex vivo epithelial organ
- Artikel/kapitelEngelska2022
Förlag, utgivningsår, omfång ...
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2022-07-19
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The Company of Biologists,2022
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electronicrdacarrier
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LIBRIS-ID:oai:DiVA.org:uu-484850
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-484850URI
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https://doi.org/10.1242/jcs.260249DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Cytosolic Ca2+ is a highly dynamic, tightly regulated and broadly conserved cellular signal. Ca2+ dynamics have been studied widely in cellular monocultures, yet organs in vivo comprise heterogeneous populations of stem and differentiated cells. Here, we examine Ca2+ dynamics in the adult Drosophila intestine, a self-renewing epithelial organ in which stem cells continuously produce daughters that differentiate into either enteroendocrine cells or enterocytes. Live imaging of whole organs ex vivo reveals that stem-cell daughters adopt strikingly distinct patterns of Ca2+ oscillations after differentiation: enteroendocrine cells exhibit single-cell Ca2+ oscillations, whereas enterocytes exhibit rhythmic, long-range Ca2+ waves. These multicellular waves do not propagate through immature progenitors (stem cells and enteroblasts), of which the oscillation frequency is approximately half that of enteroendocrine cells. Organ-scale inhibition of gap junctions eliminates Ca2+ oscillations in all cell types – even, intriguingly, in progenitor and enteroendocrine cells that are surrounded only by enterocytes. Our findings establish that cells adopt fate-specific modes of Ca2+ dynamics as they terminally differentiate and reveal that the oscillatory dynamics of different cell types in a single, coherent epithelium are paced independently.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Nguyen, Amanda
(författare)
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Marchetti, Marco
(författare)
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Du, XinXin
(författare)
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Montell, Denise J.
(författare)
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Pruitt, Beth L.
(författare)
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O’Brien, Lucy Erin
(författare)
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Uppsala universitetInstitutionen för materialvetenskap
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Cell Science: The Company of Biologists135:140021-95331477-9137
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