Search: onr:"swepub:oai:DiVA.org:uu-486665" >
Development of an E...
Development of an ELISA displaying similar reactivity with reduced and oxidized human Thioredoxin-1 (Trx1) : The plasma level of Trx1 in early onset psychosis disorders
-
- Lundberg, Mathias (author)
- Uppsala universitet,Institutionen för neurovetenskap,Soder Sjukhuset, Karolinska Inst, Dept Clin Sci & Educ, Stockholm, Sweden.;Akadem Sjukhuset, S-75185 Uppsala, Sweden.
-
- Bohman, Hannes, 1965- (author)
- Uppsala universitet,Barn- och ungdomspsykiatri,Soder Sjukhuset, Karolinska Inst, Dept Clin Sci & Educ, Stockholm, Sweden.
-
- Curbo, Sophie (author)
- Karolinska Institutet
-
show more...
-
- Mansouri, Shiva (author)
- Soder Sjukhuset, Karolinska Inst, Dept Clin Sci & Educ, Stockholm, Sweden.
-
- Agartz, Ingrid (author)
- Karolinska Inst, Ctr Psychiat Res, Dept Clin Neurosci, Stockholm, Sweden.;Univ Oslo, Inst Clin Med, KG Jebsen Ctr Psychosis Res, Div Mental Hlth & Addict,NORMENT, Oslo, Norway.;Diakonhjemmet Hosp, Dept Psychiat Res, Oslo, Norway.
-
- Arestrom, Irene (author)
- Mabtech, Nacka Strand, Sweden.
-
- Ahlborg, Niklas (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Mabtech, Nacka Strand, Sweden.;Stockholm Univ, Wennergren Inst, Dept Mol Biosci, Stockholm, Sweden.
-
show less...
-
(creator_code:org_t)
- Elsevier, 2022
- 2022
- English.
-
In: JIM - Journal of Immunological Methods. - : Elsevier. - 0022-1759 .- 1872-7905. ; 510
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- The plasma level of human thioredoxin-1 (Trx1) has been shown to be increased in various somatic diseases and psychiatric disorders. However, when comparing the reported plasma levels of Trx1, a great inter-study vari-ability, as well as variability in study outcomes of differences between patients and control subjects has been observed, ultimately limiting the possibility to make comparative analyses. Trx1 is a highly redox active protein prone to form various redox forms, e.g. dimers, oligomers or Trx1-protein complexes. We have recently shown that ELISA systems may vary in reactivity to various Trx1 redox forms. The primary aim of the present study was to develop an ELISA system with similar reactivity to various Trx1 redox forms. By evaluating a panel of novel monoclonal antibodies (mAbs), in various paired combinations, three ELISA systems were generated, with observed large variability in reactivity to various Trx1 redox forms. Importantly, an ELISA system (capture mAb MT17R6 and detection mAb MT13X3-biotin), was identified that displayed similar reactivity to oxidized and DTT reduced Trx1. The ELISA system (MT17R6/MT13X3-biotin), was subsequently used to analyze the level of Trx1 in plasma from patients (< 18 years) with early onset psychosis disorders (EOP). However, no significant (p > 0.7) difference in plasma Trx1 levels between patients with EOP (n = 23) and healthy age matched controls (HC) (n = 20) were observed. Furthermore, reliable measurement was shown to be dependent on the estab-lishment of platelet poor plasma samples, enabled by rigorous blood sample centrifugation and by efficient blocking of potentially interfering heterophilic antibodies. In conclusion, we report the design and character-ization of a Trx1 ELISA system with similar reactivity to various Trx1 redox forms. Importantly, data indicated that generated ELISA systems show large variability in reactivity to various redox forms with ultimate impact on measured levels of Trx1. Overall, results from this study suggests that future studies may be strongly improved by the use of Trx1 ELISA systems with characterized specificity to various redox forms.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Psykiatri (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Psychiatry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Thioredoxin 1
- ELISA
- Early onset psychosis disorders
- Heterophilic antibodies
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database