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Determination of me...
Determination of melatonin in human plasma with solid-phase extraction, high-performance liquid chromatography and fluorescence detection
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- Römsing, Susanne (author)
- Uppsala universitet,Kemiska institutionen
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Ulfberg, J. (author)
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Bergqvist, Yngve (author)
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(creator_code:org_t)
- 2003
- 2003
- English.
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In: Scandinavian Journal of Clinical and Laboratory Investigation. - 0036-5513 .- 1502-7686. ; 63:1, s. 81-88
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- A new bioanalytical method for the determination of melatonin in plasma with high-performance liquid chromatography (HPLC) and fluorescence detection preceded by solid-phase extraction has been developed and validated. Melatonin was extracted from 3 mL plasma using a Waters Oasis HLB solid-phase extraction cartridge and the elute was evaporated to dryness and dissolved in 200 microl mobile phase; acetonitrile-phosphate buffer, 0.01 M pH 7.2 (25:75, v/v). 125 microL was injected into the HPLC system and separation was carried out on a Waters SymmetryShield RP18 column 5 microm (250 x 4.6 mm). Excitation and emission wavelengths were set to 285 nm and 345 nm, respectively. The HPLC system was able to separate melatonin and internal standard (5-fluorotryptamine) from other endogenous indole compounds such as serotonin and tryptophan. Determination down to 0.10 nmol/L was possible, with an intra-assay precision of about 13%. Melatonin was stable in plasma for at least 30 days at about 23 degrees C.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Analytical chemistry
- Analytisk kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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