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Interacting polymer...
Interacting polymer-modification enzymes in heparan sulfate biosynthesis
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- Zhang, Tianji (author)
- Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.;Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China.
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- Yu, Mingjia (author)
- Beijing Inst Technol, Sch Chem & Chem Engn, Beijing, Peoples R China.;Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing, Peoples R China.
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- Li, Honglian (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Maccarana, Marco (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Zhang, Wei (author)
- Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.
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- Shi, Deling (author)
- Shandong Univ, Natl Glycoengn Res Ctr, Jinan, Peoples R China.
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- Kan, Ying (author)
- Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China.
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- Zhang, Xiao (author)
- Uppsala universitet,Forskargrupp Dan Larhammar
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- Chi, Lianli (author)
- Shandong Univ, Natl Glycoengn Res Ctr, Jinan, Peoples R China.
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- Lindahl, Ulf (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Li, Hongmei (author)
- Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China.
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- Li, Jin-Ping (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing, Peoples R China.
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- Tan, Tianwei (author)
- Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China.
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Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China;Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China. Beijing Inst Technol, Sch Chem & Chem Engn, Beijing, Peoples R China.;Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing, Peoples R China. (creator_code:org_t)
- Elsevier, 2023
- 2023
- English.
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In: Carbohydrate Polymers. - : Elsevier. - 0144-8617 .- 1879-1344. ; 299
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues. Incubations of a [4GlcA beta 1-4GlcNSO3 alpha 1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotrans-ferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps. Enzyme complexes were supported by computational modeling and homogeneous time resolved fluores-cence. GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions. Evidence for a func-tional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O -sulfated glucosamine residues. The inability to achieve simultaneous 2-O-and 6-O-sulfation in vitro supported topologically separated reactions in the cell. These findings provide novel insight into the roles of enzyme in-teractions in heparan sulfate biosynthesis.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Heparan sulfate
- Glucuronosyl C5-epimerase
- 2-O-sulfotransferase
- 6-Osulfotransferase
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Zhang, Tianji
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Yu, Mingjia
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Li, Honglian
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Maccarana, Marco
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Zhang, Wei
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Shi, Deling
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show more...
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Kan, Ying
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Zhang, Xiao
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Chi, Lianli
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Lindahl, Ulf
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Li, Hongmei
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Li, Jin-Ping
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Tan, Tianwei
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Carbohydrate Pol ...
- By the university
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Uppsala University