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The β-cell primary ...
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Sanchez, GonzaloUppsala universitet,Institutionen för medicinsk cellbiologi
(author)
The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling
- Article/chapterEnglish2023
Publisher, publication year, extent ...
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2022-11-09
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Rockefeller University Press,2023
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-492139
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-492139URI
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https://doi.org/10.1083/jcb.202108101DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The primary cilium is an organelle present in most adult mammalian cells that is considered as an antenna for sensing the local microenvironment. Here, we use intact mouse pancreatic islets of Langerhans to investigate signaling properties of the primary cilium in insulin-secreting β-cells. We find that GABAB1 receptors are strongly enriched at the base of the cilium, but are mobilized to more distal locations upon agonist binding. Using cilia-targeted Ca2+ indicators, we find that activation of GABAB1 receptors induces selective Ca2+ influx into primary cilia through a mechanism that requires voltage-dependent Ca2+ channel activation. Islet β-cells utilize cytosolic Ca2+ increases as the main trigger for insulin secretion, yet we find that increases in cytosolic Ca2+ fail to propagate into the cilium, and that this isolation is largely due to enhanced Ca2+ extrusion in the cilium. Our work reveals local GABA action on primary cilia that involves Ca2+ influx and depends on restricted Ca2+ diffusion between the cilium and cytosol.
Subject headings and genre
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Incedal, Tugce CerenUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)cerin971
(author)
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Prada, Juan
(author)
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O'Callaghan, PaulUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)pauoo824
(author)
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Dyachok, Oleg,1965-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)olegdyac
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Echeverry, SantiagoUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)sanec726
(author)
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Dumral, ÖzgeUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)ozgdu393
(author)
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Nguyen, Phuoc MyUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)phung164
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Xie, BeichenUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)beixi713
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Barg, Sebastian,1969-Uppsala universitet,Institutionen för medicinsk cellbiologi,Barg(Swepub:uu)sebba507
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Kreuger, Johan,1972-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)jkr07842
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Dandekar, Thomas
(author)
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Idevall Hagren, Olof,1980-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)olide169
(author)
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Uppsala universitetInstitutionen för medicinsk cellbiologi
(creator_code:org_t)
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In:Journal of Cell Biology: Rockefeller University Press222:10021-95251540-8140
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Sanchez, Gonzalo
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Incedal, Tugce C ...
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Prada, Juan
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O'Callaghan, Pau ...
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Dyachok, Oleg, 1 ...
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Echeverry, Santi ...
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Dumral, Özge
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Nguyen, Phuoc My
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Xie, Beichen
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Barg, Sebastian, ...
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Kreuger, Johan, ...
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Dandekar, Thomas
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Idevall Hagren, ...
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- MEDICAL AND HEALTH SCIENCES
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