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Silencing DTX3L Inh...
Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway
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- Hu, Wei (author)
- Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300350, Peoples R China.;Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.
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- Hu, Yaorui (author)
- Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.;Binzhou Med Univ, Sch Basic Med, Yantai 264000, Peoples R China.
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- Pei, Yao (author)
- Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.
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- Li, Rongrong (author)
- Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.;Binzhou Med Univ, Sch Basic Med, Yantai 264000, Peoples R China.
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- Xu, Fuyi (author)
- Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.
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- Chi, Xiaodong (author)
- Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.
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- Mi, Jia (author)
- Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.
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- Bergquist, Jonas (author)
- Uppsala universitet,Analytisk kemi,Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.
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- Lu, Lu (author)
- Univ Tennessee, Dept Genet Genom & Informat, Hlth Sci Ctr, Memphis, TN 38163 USA.
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- Zhang, Luping (author)
- Binzhou Med Univ, Sch Basic Med, Yantai 264000, Peoples R China.
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- Yang, Chunhua (author)
- Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.
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Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300350, Peoples R China;Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China. Binzhou Med Univ, Shandong Technol Innovat Ctr Mol Targeting & Intel, Sch Pharm, Yantai 264000, Peoples R China.;Binzhou Med Univ, Sch Basic Med, Yantai 264000, Peoples R China. (creator_code:org_t)
- 2023-01-03
- 2023
- English.
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 24:1
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https://uu.diva-port... (primary) (Raw object)
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Abstract
Subject headings
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- Cervical carcinoma (CC) is the second most prevalent gynecologic cancer in females across the world. To obtain a better understanding of the mechanisms underlying the development of CC, high-resolution label-free mass spectrometry was performed on CC and adjacent normal tissues from eight patients. A total of 2631 proteins were identified, and 46 significant differently expressed proteins (DEPs) were found between CC and normal tissues (p < 0.01, fold change >10 or <0.1). Ingenuity pathway analysis revealed that the majority of the proteins were involved in the regulation of eIF4 and p70S6K signaling and mTOR signaling. Among 46 DEPs, Integrin beta 6 (ITGB6), PPP1CB, TMPO, PTGES3 (P23) and DTX3L were significantly upregulated, while Desmin (DES) was significantly downregulated in CC tissues compared with the adjacent normal tissues. In in vivo and in vitro experiments, DTX3L knockdown suppressed CC cell proliferation, migration, invasion and xenograft tumorigenesis, and enhanced cell apoptosis. Combination of silencing DTX3L and cisplatin treatment induced higher apoptosis percentage compared to cisplatin treatment alone. Moreover, DTX3L silencing inhibited the PI3K/AKT/mTOR signal pathway. Thus, our results suggested DTX3L could regulate CC progression through the PI3K/AKT/mTOR signal pathway and is potentially a novel biomarker and therapeutic target for CC.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- cervical carcinoma (CC)
- Deltex-3-like
- PI3K
- AKT
- mTOR signaling pathway
- therapeutic target
- quantitative proteomics
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Hu, Wei
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Hu, Yaorui
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Pei, Yao
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Li, Rongrong
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Xu, Fuyi
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Chi, Xiaodong
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show more...
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Mi, Jia
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Bergquist, Jonas
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Lu, Lu
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Zhang, Luping
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Yang, Chunhua
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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International Jo ...
- By the university
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Uppsala University