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Urocortin3-expressi...
Urocortin3-expressing neurons in sensory transmission
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- Franck, Marina Christina Mikaela (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Malin Lagerström
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- Weman, Hannah M., 1993- (author)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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- Ceder, Mikaela M., 1991- (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Genomik och neurobiologi,Malin Lagerström
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- Ahemaiti, Aikeremu, 1984- (author)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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- Ma, Haisha (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi
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Bengtsson, Erica (author)
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- Magnusson, Kajsa A., 1997- (author)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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- Koning, Harmen Kornelis, 1991- (author)
- Henrik Boije
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- Öhman, Caroline (author)
- Uppsala universitet,Tillämpad materialvetenskap
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- Lagerström, Malin C., 1977- (author)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- Urocortin 3 (UCN3) is a neuropeptide involved in mechanosensation and stress regulation, and Ucn3-Cre neurons have been assigned a role in mechanical itch. Here, we show that Ucn3 marks a population of excitatory neurons in the mouse dorsal horn, divided into two non-overlapping subpopulations expressing protein kinase C g or calretinin/calbindin 2. Chemogenetic activation of spinal Ucn3-Cre neurons evoked a targeted biting/licking behavior towards the corresponding dermatome. Genetic deletion of vesicular glutamate transporter 2 (VGLUT2) in Ucn3-Cre neurons removed the phenotype, showing that the biting/licking behavior is VGLUT2-dependent. Conditional deletion of VGLUT2 did not affect acute thermal or mechanical withdrawal responses, nor thermal withdrawal responses after nerve growth factor-induced hypersensitivity or the prurifensive response to 48/80 or von Frey stimuli applied in nape. Instead, we found that a group of spinal Ucn3 neurons were activated in response to artificial scratching or 48/80-induced itch. Electrophysiological experiments showed that spinal Ucn3 neurons received both glycinergic and GABAergic tonic inhibition, and monosynaptic inputs from both Aβ and C fibers, which could be confirmed by rabies tracing. Spinal Ucn3/Ucn3-Cre neurons thus represent a mechanically sensitive population with several roles in the itch-scratch cycle.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Urocortin 3
- spinal cord
- itch
- vesicular glutamate transporter 2
- retrograde rabies tracing
- pharmacogenetics
Publication and Content Type
- vet (subject category)
- ovr (subject category)
To the university's database
- By the author/editor
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Franck, Marina C ...
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Weman, Hannah M. ...
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Ceder, Mikaela M ...
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Ahemaiti, Aikere ...
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Ma, Haisha
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Bengtsson, Erica
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show more...
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Magnusson, Kajsa ...
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Koning, Harmen K ...
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Öhman, Caroline
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Lagerström, Mali ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- By the university
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Uppsala University