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A Mitosome With Dis...
A Mitosome With Distinct Metabolism in the Uncultured Protist Parasite Paramikrocytos canceri (Rhizaria, Ascetosporea)
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- Onuţ-Brännström, Ioana (author)
- Uppsala University,Uppsala universitet,Systematisk biologi
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- Stairs, Courtney W (author)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologigruppen,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Microbiology Group,Lund University Research Groups
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- Campos, Karla Iveth Aguilera (author)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologigruppen,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Microbiology Group,Lund University Research Groups
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- Hiltunen Thorén, Markus (author)
- Uppsala University,Uppsala universitet,Systematisk biologi
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- Ettema, Thijs J. G. (author)
- Wageningen Univ & Res, Lab Microbiol, Wageningen, Netherlands.,Wageningen University
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- Keeling, Patrick J. (author)
- Univ British Columbia, Dept Bot, Vancouver, BC, Canada.,University of British Columbia
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- Bass, David (author)
- Ctr Environm Fisheries & Aquaculture Sci Cefas, Int Ctr Excellence Aquat Anim Hlth, Weymouth, England.;Nat Hist Museum, Dept Life Sci, London, England.;Univ Exeter, Coll Life & Environm Sci, Sustainable Aquaculture Futures, Biosci, Exeter, England.,International Centre of Excellence for Aquatic Animal Health (CEFAS)
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- Burki, Fabien (author)
- Uppsala University,Uppsala universitet,Systematisk biologi,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2023-02-15
- 2023
- English.
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In: Genome Biology and Evolution. - : Oxford University Press. - 1759-6653. ; 15:3
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Abstract
Subject headings
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- Ascetosporea are endoparasites of marine invertebrates that include economically important pathogens of aquaculture species. Owing to their often-minuscule cell sizes, strict intracellular lifestyle, lack of cultured representatives and minimal availability of molecular data, these unicellular parasites remain poorly studied. Here, we sequenced and assembled the genome and transcriptome of Paramikrocytos canceri, an endoparasite isolated from the European edible crab Cancer pagurus. Using bioinformatic predictions, we show that P. canceri likely possesses a mitochondrion-related organelle (MRO) with highly reduced metabolism, resembling the mitosomes of other parasites but with key differences. Like other mitosomes, this MRO is predicted to have reduced metabolic capacity and lack an organellar genome and function in iron–sulfur cluster (ISC) pathway-mediated Fe–S cluster biosynthesis. However, the MRO in P. canceri is uniquely predicted to produce ATP via a partial glycolytic pathway and synthesize phospholipids de novo through the CDP-DAG pathway. Heterologous gene expression confirmed that proteins from the ISC and CDP-DAG pathways retain mitochondrial targeting sequences that are recognized by yeast mitochondria. This represents a unique combination of metabolic pathways in an MRO, including the first reported case of a mitosome-like organelle able to synthesize phospholipids de novo. Some of these phospholipids, such as phosphatidylserine, are vital in other protist endoparasites that invade their host through apoptotic mimicry.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- mitochondrial evolution
- mitosome
- mikrocytids
- Paramikrocytos canceri
- Ascetosporea
- rhizaria
- protists
- parasites
- Animals
- Parasites
- Rhizaria/genetics
- Organelles
- Mitochondria/genetics
- Saccharomyces cerevisiae/metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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