Search: onr:"swepub:oai:DiVA.org:uu-503252" >
OptoRheo :
OptoRheo : Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems
-
- Mendonca, Tania (author)
- Univ Nottingham, Fac Engn, Opt & Photon Res Grp, Nottingham, England.
-
- Lis-Slimak, Katarzyna (author)
- Univ Nottingham, Nottingham Biodiscovery Inst, Sch Med, Nottingham, England.
-
- Matheson, Andrew B. (author)
- Heriot Watt Univ, Inst Biol Chem Biophys & Bioengn, Sch Engn & Phys Sci, Edinburgh, Scotland.
-
show more...
-
- Smith, Matthew G. (author)
- Univ Glasgow, James Watt Sch Engn, Div Biomed Engn, Glasgow, Scotland.
-
- Anane-Adjei, Akosua B. (author)
- Univ Nottingham, Sch Pharm, Nottingham, England.
-
- Ashworth, Jennifer C. (author)
- Univ Nottingham, Nottingham Biodiscovery Inst, Sch Med, Nottingham, England.;Univ Nottingham, Sch Vet Med & Sci, Sutton Bonington Campus, Leicestershire, England.
-
- Cavanagh, Robert (author)
- Univ Nottingham, Sch Pharm, Nottingham, England.
-
- Paterson, Lynn (author)
- Heriot Watt Univ, Inst Biol Chem Biophys & Bioengn, Sch Engn & Phys Sci, Edinburgh, Scotland.
-
- Dalgarno, Paul A. (author)
- Heriot Watt Univ, Inst Biol Chem Biophys & Bioengn, Sch Engn & Phys Sci, Edinburgh, Scotland.
-
- Alexander, Cameron (author)
- Univ Nottingham, Sch Pharm, Nottingham, England.
-
- Tassieri, Manlio (author)
- Univ Glasgow, James Watt Sch Engn, Div Biomed Engn, Glasgow, Scotland.
-
- Merry, Catherine L. R. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Univ Nottingham, Nottingham Biodiscovery Inst, Sch Med, Nottingham, England.;
-
- Wright, Amanda J. (author)
- Univ Nottingham, Fac Engn, Opt & Photon Res Grp, Nottingham, England.
-
show less...
-
Univ Nottingham, Fac Engn, Opt & Photon Res Grp, Nottingham, England Univ Nottingham, Nottingham Biodiscovery Inst, Sch Med, Nottingham, England. (creator_code:org_t)
- Springer Nature, 2023
- 2023
- English.
-
In: Communications Biology. - : Springer Nature. - 2399-3642. ; 6:1
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- A new instrument named OptoRheo combines light sheet fluorescence microscopy and particle tracking microrheology for live imaging and micromechanical sensing of extracellular matrix-cell interactions. Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument named 'OptoRheo' that combines light sheet fluorescence microscopy with particle tracking microrheology. OptoRheo lets us image cells in 3D as they proliferate over several days while simultaneously sensing the mechanical properties of the surrounding extracellular and pericellular matrix at a sub-cellular length scale. OptoRheo can be used in two operational modalities (with and without an optical trap) to extend the dynamic range of microrheology measurements. We corroborated this by characterising the ECM surrounding live breast cancer cells in two distinct culture systems, cell clusters in 3D hydrogels and spheroids in suspension culture. This cutting-edge instrument will transform the exploration of drug transport through complex cell culture matrices and optimise the design of the next-generation of disease models.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Mendonca, Tania
-
Lis-Slimak, Kata ...
-
Matheson, Andrew ...
-
Smith, Matthew G ...
-
Anane-Adjei, Ako ...
-
Ashworth, Jennif ...
-
show more...
-
Cavanagh, Robert
-
Paterson, Lynn
-
Dalgarno, Paul A ...
-
Alexander, Camer ...
-
Tassieri, Manlio
-
Merry, Catherine ...
-
Wright, Amanda J ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biophysics
- Articles in the publication
-
Communications B ...
- By the university
-
Uppsala University