Opioid effects on 45Ca2+ uptake and glutamate release in rat cerebral cortex in primary culture

Primary cultures of rat cortex, conveniently prepared from newborn animals, were used to study opioid effects on 45Ca2+ uptake and glutamate release. 45Ca2+ uptake, induced by treatment with glutamate or NMDA, was largely blocked by the NMDA antagonist MK-801. K+ depolarization-induced 45Ca2+ uptake was also reduced by MK-801, indicating that the effect was mediated by glutamate release. Direct analysis verified that glutamate, and aspartate, were indeed released. Opioid peptides of the prodynorphin system were also released and these, or other peptides, were functionally active, because naloxone treatment increased glutamate release, as well as the 45Ca2+ uptake induced by depolarization. Opioid agonists, selective for mu-, kappa-, and delta-receptors, inhibited the 45Ca2+ uptake induced by K+ depolarization. The combination of low concentrations of MK-801 and opioid agonists resulted in additive inhibition of K(+)-induced 45Ca2+ uptake. The results indicate that this system may be useful as an in vitro CNS model for studying modulation by opioids of glutamate release and Ca2+ uptake under acute, and perhaps also chronic, opiate treatment.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmaceutiska vetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmaceutical Sciences hsv//eng
primary culture
rat cerebral cortex
dynorphin peptides
glutamate release
Ca-45(2+) uptake
opiate agonists
naloxone
MEDICINE
MEDICIN
PHARMACY
FARMACI

Schramm, Michael (author)
Nylander, IngridDepartment of Clinical Neuroscience, Experimental Alcohol and Drug Addiction Research Section, Karolinska Institute,Pharmacology(Swepub:uu)iny08577 (author)
Kasakov, Lubomir (author)
You, Zhi-Bing (author)
Herrera-Marschitz, Mario (author)
Terenius, LarsKarolinska Institutet (author)
HerreraMarschitz, M (author)
Department of Clinical Neuroscience, Experimental Alcohol and Drug Addiction Research Section, Karolinska InstitutePharmacology (creator_code:org_t)

By the author/editor: Vlaskovska, Mila; Schramm, Michael; Nylander, Ingrid; Kasakov, Lubomir; You, Zhi-Bing; Herrera-Marschit ...; show more...; Terenius, Lars; HerreraMarschitz ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmaceutical S ...

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