Structure and its transformation of elliptical nege- like virus Tanay virus

Okamoto, Kenta (author): Uppsala universitet,Molekylär biofysik,Science for Life Laboratory, SciLifeLab

Song, Chihong (author): Natl Inst Nat Sci NINS, Natl Inst Physiol Sci NIPS, Okazaki, Aichi, Japan.;Natl Inst Nat Sci NINS, Exploratory Res Ctr Life & Living Syst ExCELLS, Tokyo, Japan.;Pusan Natl Univ, Core Res Facil, Yangsan 50612, South Korea

Sakaguchi, Miako (author): Nagasaki Univ, Inst Trop Med NEKKEN, Cent Lab, Nagasaki, Japan

Chalkiadaki, Christina (author): Uppsala universitet,Institutionen för cell- och molekylärbiologi

Miyazaki, Naoyuki (author): Natl Inst Nat Sci NINS, Natl Inst Physiol Sci NIPS, Okazaki, Aichi, Japan

Nabeshima, Takeshi (author): Nagasaki Univ, Inst Trop Med NEKKEN, Dept Virol, Nagasaki, Japan

Morita, Kouichi (author): Nagasaki Univ, Inst Trop Med NEKKEN, Dept Virol, Nagasaki, Japan

Inoue, Shingo (author): Nagasaki Univ, Inst Trop Med NEKKEN, Dept Virol, Nagasaki, Japan

Murata, Kazuyoshi (author): Natl Inst Nat Sci NINS, Natl Inst Physiol Sci NIPS, Okazaki, Aichi, Japan.;Natl Inst Nat Sci NINS, Exploratory Res Ctr Life & Living Syst ExCELLS, Tokyo, Japan

(creator_code:org_t)

Microbiology Society, 2023
2023
English.
In: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 104:6

Related links:: https://doi.org/10.1...; show more...; https://uu.diva-port... (primary) (Raw object); https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

Negeviruses that infect insects are recently identified virus species that are phylogenetically related to several plant viruses. They exhibit a unique virion structure, an elliptical core with a short projection. Negeviruses encode two structural proteins, a glycoprotein that forms a short projection, and an envelope protein that forms an elliptical core. The glycoprotein has been reported only in the negeviruses' genes, and not in phylogenetically related plant viruses' genes. In this report, we first describe the three- dimensional electron cryo- microscopy (cryo- EM) structure of Tanay virus (TANAV), one of the nege- like viruses. TANAV particle demonstrates a periodical envelope structure consisting of three layers surrounding the centred viral RNA. The elliptical core dynamically changes its shape under acidic and even low detergent conditions to form bullet- like or tubular shapes. The further cryo- EM studies on these transformed TANAV particles reveal their overall structural rearrangement. These findings suggest putative geometries of TANAV and its transformation in the life cycle, and the potential importance of the short projection for enabling cell entry to the insect hosts.

By the author/editor: Okamoto, Kenta; Song, Chihong; Wang, Han; Sakaguchi, Miako; Chalkiadaki, Chr ...; Miyazaki, Naoyuk ...; show more...; Nabeshima, Takes ...; Morita, Kouichi; Inoue, Shingo; Murata, Kazuyosh ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.