onr:"swepub:oai:DiVA.org:uu-509282"
Search: onr:"swepub:oai:DiVA.org:uu-509282" > Screening autism-as...
- 1 of 1
- Previous record
- Next record
- To hitlist
Screening autism-associated environmental factors in differentiating human neural progenitors with fractional factorial design-based transcriptomics
-
- Arora, Abishek (author)
- Karolinska Institute,Karolinska Institutet,Karolinska Inst, Ctr Neurodev Disorders KIND, Ctr Psychiat Res, Dept Womens & Childrens Hlth, BioClin J9:30,Visionsgatan 4, S-17156 Solna, Stockholm, Sweden.;Karolinska Univ Hosp, Astrid Lindgren Childrens Hosp, Stockholm, Region Stockhol, Sweden.,Karolinska University Hospital
-
- Becker, Martin (author)
- Karolinska Institute,Karolinska University Hospital
-
- Marques-Santos, Cátia M. (author)
- Uppsala University,Uppsala universitet,Institutionen för kemi - BMC
- show more...
- show less...
- Karolinska Institutet Karolinska Inst, Ctr Neurodev Disorders KIND, Ctr Psychiat Res, Dept Womens & Childrens Hlth, BioClin J9:30,Visionsgatan 4, S-17156 Solna, Stockholm, Sweden;Karolinska Univ Hosp, Astrid Lindgren Childrens Hosp, Stockholm, Region Stockhol, Sweden. (creator_code:org_t)
- Springer Nature, 2023
- 2023
- English.
- In: Scientific Reports. - : Springer Nature. - 2045-2322. ; 13
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Research continues to identify genetic variation, environmental exposures, and their mixtures underlying different diseases and conditions. There is a need for screening methods to understand the molecular outcomes of such factors. Here, we investigate a highly efficient and multiplexable, fractional factorial experimental design (FFED) to study six environmental factors (lead, valproic acid, bisphenol A, ethanol, fluoxetine hydrochloride and zinc deficiency) and four human induced pluripotent stem cell line derived differentiating human neural progenitors. We showcase the FFED coupled with RNA-sequencing to identify the effects of low-grade exposures to these environmental factors and analyse the results in the context of autism spectrum disorder (ASD). We performed this after 5-day exposures on differentiating human neural progenitors accompanied by a layered analytical approach and detected several convergent and divergent, gene and pathway level responses. We revealed significant upregulation of pathways related to synaptic function and lipid metabolism following lead and fluoxetine exposure, respectively. Moreover, fluoxetine exposure elevated several fatty acids when validated using mass spectrometry-based metabolomics. Our study demonstrates that the FFED can be used for multiplexed transcriptomic analyses to detect relevant pathway-level changes in human neural development caused by low-grade environmental risk factors. Future studies will require multiple cell lines with different genetic backgrounds for characterising the effects of environmental exposures in ASD.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Scientific Reports (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Arora, Abishek
- Becker, Martin
- Marques-Santos, ...
- Oksanen, Marika
- Li, Danyang
- Mastropasqua, Fr ...
- show more...
- Watts, Michelle ...
- Arora, Manish
- Falk, Anna
- Daub, Carsten Ol ...
- Lanekoff, Ingela ...
- Tammimies, Krist ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
- Articles in the publication
- Scientific Repor ...
- By the university
- Uppsala University
- Karolinska Institutet
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
