onr:"swepub:oai:DiVA.org:uu-511381"
Search: onr:"swepub:oai:DiVA.org:uu-511381" > Dynamics of release...
- 1 of 1
- Previous record
- Next record
- To hitlist
Dynamics of release factor recycling during translation termination in bacteria
-
- Prabhakar, Arjun (author)
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Program Biophys, Stanford, CA USA.;Pacific Biosci Inc, Menlo Pk, CA 94025 USA
-
- Pavlov, Michael Y. (author)
- Uppsala universitet,Molekylärbiologi
-
- Zhang, Jingji (author)
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA
- show more...
- show less...
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA;Stanford Univ, Program Biophys, Stanford, CA USA.;Pacific Biosci Inc, Menlo Pk, CA 94025 USA Molekylärbiologi (creator_code:org_t)
- Oxford University Press, 2023
- 2023
- English.
- In: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 51:11, s. 5774-5790
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- In bacteria, release of newly synthesized proteins from ribosomes during translation termination is catalyzed by class-I release factors (RFs) RF1 or RF2, reading UAA and UAG or UAA and UGA codons, respectively. Class-I RFs are recycled from the post-termination ribosome by a class-II RF, the GTPase RF3, which accelerates ribosome intersubunit rotation and class-I RF dissociation. How conformational states of the ribosome are coupled to the binding and dissociation of the RFs remains unclear and the importance of ribosome-catalyzed guanine nucleotide exchange on RF3 for RF3 recycling in vivo has been disputed. Here, we profile these molecular events using a single-molecule fluorescence assay to clarify the timings of RF3 binding and ribosome intersubunit rotation that trigger class-I RF dissociation, GTP hydrolysis, and RF3 dissociation. These findings in conjunction with quantitative modeling of intracellular termination flows reveal rapid ribosome-dependent guanine nucleotide exchange to be crucial for RF3 action in vivo.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Nucleic Acids Research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Prabhakar, Arjun
- Pavlov, Michael ...
- Zhang, Jingji
- Indrisiunaite, G ...
- Wang, Jinfan
- Lawson, Michael ...
- show more...
- Ehrenberg, Måns
- Puglisi, Joseph ...
- show less...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Biochemistry and ...
- Articles in the publication
- Nucleic Acids Re ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
