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Guidelines for micr...
Guidelines for microbiome studies in renal physiology
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- Muralitharan, Rikeish R. (author)
- Monash Univ, Fac Sci, Sch Biol Sci, Hypertens Res Lab, Melbourne, Vic, Australia.;Minist Hlth Malaysia, Inst Med Res, Kuala Lumpur, Malaysia.
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- Snelson, Matthew (author)
- Monash Univ, Cent Clin Sch, Dept Diabet, Melbourne, Vic, Australia.
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- Meric, Guillaume (author)
- Uppsala universitet,Molekylär epidemiologi,Science for Life Laboratory, SciLifeLab,Baker Heart & Diabet Inst, Cambridge Baker Syst Genom Initiat, Melbourne, Vic, Australia.;Univ Melbourne, Dept Cardiometab Hlth, Melbourne, Vic, Australia.;La Trobe Univ, Dept Cardiovasc Res Translat & Implementat, Melbourne, Vic, Australia.
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- Coughlan, Melinda T. (author)
- Monash Univ, Cent Clin Sch, Dept Diabet, Melbourne, Vic, Australia.;Monash Inst Pharmaceut Sci, Drug Discovery Biol, Parkville, Vic, Australia.
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- Marques, Francine Z. (author)
- Monash Univ, Fac Sci, Sch Biol Sci, Hypertens Res Lab, Melbourne, Vic, Australia.;Baker Heart & Diabet Inst, Heart Failure Res Grp, Melbourne, Vic, Australia.;Monash Univ, Victorian Heart Inst, Melbourne, Vic, Australia.
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Monash Univ, Fac Sci, Sch Biol Sci, Hypertens Res Lab, Melbourne, Vic, Australia;Minist Hlth Malaysia, Inst Med Res, Kuala Lumpur, Malaysia. Monash Univ, Cent Clin Sch, Dept Diabet, Melbourne, Vic, Australia. (creator_code:org_t)
- American Physiological Society, 2023
- 2023
- English.
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In: American Journal of Physiology - Renal Physiology. - : American Physiological Society. - 1931-857X .- 1522-1466. ; 325:3, s. F345-F362
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Gut microbiome research has increased dramatically in the last decade, including in renal health and disease. The field is moving from experiments showing mere association to causation using both forward and reverse microbiome approaches, leveraging tools such as germ-free animals, treatment with antibiotics, and fecal microbiota transplantations. However, we are still seeing a gap between discovery and translation that needs to be addressed, so that patients can benefit from microbiome-based therapies. In this guideline paper, we discuss the key considerations that affect the gut microbiome of animals and clinical studies assessing renal function, many of which are often overlooked, resulting in false-positive results. For animal studies, these include suppliers, acclimatization, baseline microbiota and its normalization, littermates and cohort/cage effects, diet, sex differences, age, circadian differences, antibiotics and sweeteners, and models used. Clinical studies have some unique considerations, which include sampling, gut transit time, dietary records, medication, and renal phenotypes. We provide best-practice guidance on sampling, storage, DNA extraction, and methods for microbial DNA sequencing (both 16S rRNA and shotgun metagenome). Finally, we discuss follow-up analyses, including tools available, metrics, and their interpretation, and the key challenges ahead in the microbiome field. By standardizing study designs, methods, and reporting, we will accelerate the findings from discovery to translation and result in new microbiome-based therapies that may improve renal health.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- 16S
- germ-free
- kidney
- metagenome
- microbiome
Publication and Content Type
- ref (subject category)
- for (subject category)
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