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Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of Leguminosae plants
- Article/chapterEnglish2023
Publisher, publication year, extent ...
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Springer Nature,2023
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electronicrdacarrier
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LIBRIS-ID:oai:DiVA.org:uu-515564
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-515564URI
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https://doi.org/10.1038/s41467-023-42393-1DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Apiose is a natural pentose containing an unusual branched-chain structure. Apiosides are bioactive natural products widely present in the plant kingdom. However, little is known on the key apiosylation reaction in the biosynthetic pathways of apiosides. In this work, we discover an apiosyltransferase GuApiGT from Glycyrrhiza uralensis. GuApiGT could efficiently catalyze 2″-O-apiosylation of flavonoid glycosides, and exhibits strict selectivity towards UDP-apiose. We further solve the crystal structure of GuApiGT, determine a key sugar-binding motif (RLGSDH) through structural analysis and theoretical calculations, and obtain mutants with altered sugar selectivity through protein engineering. Moreover, we discover 121 candidate apiosyltransferase genes from Leguminosae plants, and identify the functions of 4 enzymes. Finally, we introduce GuApiGT and its upstream genes into Nicotiana benthamiana, and complete de novo biosynthesis of a series of flavonoid apiosides. This work reports an efficient phenolic apiosyltransferase, and reveals mechanisms for its sugar donor selectivity.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Wang, Zi-Long
(author)
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Chen, Kuan
(author)
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Yao, Ming-Ju
(author)
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Zhang, Meng
(author)
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Wang, Rong-Shen
(author)
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Zhang, Jia-He
(author)
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Ågren, HansUppsala universitet,Kemisk och biomolekylär fysik(Swepub:uu)hanag788
(author)
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Li, Fu-Dong
(author)
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Li, JunhaoUppsala universitet,Kemisk och biomolekylär fysik(Swepub:uu)jonli333
(author)
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Qiao, Xue
(author)
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Ye, Min
(author)
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Uppsala universitetKemisk och biomolekylär fysik
(creator_code:org_t)
Related titles
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In:Nature Communications: Springer Nature14:12041-1723
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Wang, Hao-Tian
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Wang, Zi-Long
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Chen, Kuan
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Yao, Ming-Ju
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Zhang, Meng
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Wang, Rong-Shen
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Zhang, Jia-He
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Ågren, Hans
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Li, Fu-Dong
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Li, Junhao
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Qiao, Xue
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Ye, Min
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- NATURAL SCIENCES
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NATURAL SCIENCES
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Uppsala University