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Immature neurons fr...
Immature neurons from CNS stem cells proliferate in response toplatelet-derived growth factor
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- Erlandsson, Anna (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Enarsson, Maria (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Forsberg-Nilsson, Karin (author)
- Uppsala universitet,Institutionen för genetik och patologi
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: Journal of Neuroscience. - 0270-6474 .- 1529-2401. ; 21:10, s. 3483-3491
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Abstract
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- Identifying external signals involved in the regulation of neural stem cell proliferation and differentiation is fundamental to the understanding of CNS development. In this study we show that platelet-derived growth factor (PDGF) can act as a mitogen for neural precursor cells. Multipotent stem cells from developing CNS can be maintained in a proliferative state under serum-free conditions in the presence of fibroblast growth factor-2 (FGF2) and induced to differentiate into neurons, astrocytes, and oligodendrocytes on withdrawal of the mitogen. PDGF has been suggested to play a role during the differentiation into neurons. We have investigated the effect of PDGF on cultured stem cells from embryonic rat cortex. The PDGF alpha-receptor is constantly expressed during differentiation of neural stem cells but is phosphorylated only after PDGF-AA treatment. In contrast, the PDGF beta-receptor is hardly detectable in uncommitted cells, but its expression increases during differentiation. We show that PDGF stimulation leads to c-fos induction, 5'-bromo-2'deoxyuridine incorporation, and an increase in the number of immature cells stained with antibodies to neuronal markers. Our findings suggest that PDGF acts as a mitogen in the early phase of stem cell differentiation to expand the pool of immature neurons.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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