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Exploring the epide...
Exploring the epidemiology of mcr genes, genetic context and plasmids in Enterobacteriaceae originating from pigs and humans on farms in Thailand
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- Leangapichart, Thongpan (author)
- Section for Food Safety and Animal Health Research, Norwegian Veterinary Institute , Ås , Norway
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- Stosic, Milan S (author)
- Section for Food Safety and Animal Health Research, Norwegian Veterinary Institute , Ås , Norway
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- Hickman, Rachel A. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Infektionsmedicin
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- Lunha, Kamonwan (author)
- Department of Clinical Sciences, Swedish University of Agricultural Sciences , Uppsala , Sweden
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- Jiwakanon, Jatesada (author)
- Research Group for Animal Health Technology, Faculty of Veterinary Medicine, Khon Kaen University , Khon Kaen , Thailand
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- Angkititrakul, Sunpetch (author)
- Research Group for Animal Health Technology, Faculty of Veterinary Medicine, Khon Kaen University , Khon Kaen , Thailand
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- Magnusson, Ulf (author)
- Department of Clinical Sciences, Swedish University of Agricultural Sciences , Uppsala , Sweden
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- Van Boeckel, Thomas P (author)
- Health Geography and Policy Group, ETH Zurich , Zurich , Switzerland;Center for Diseases Dynamics Economics & Policy , Washington, DC , USA
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- Järhult, Josef D., 1975- (author)
- Uppsala universitet,Infektionsmedicin,Institutionen för medicinsk biokemi och mikrobiologi
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- Sunde, Marianne (author)
- Section for Food Safety and Animal Health Research, Norwegian Veterinary Institute , Ås , Norway
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(creator_code:org_t)
- Oxford University Press, 2023
- 2023
- English.
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In: Journal of Antimicrobial Chemotherapy. - : Oxford University Press. - 0305-7453 .- 1460-2091. ; 78:6, s. 1395-1405
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Abstract
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- Objectives In veterinary medicine, colistin has been widely used as therapeutic and prophylactic agent, and for growth promotion. However, colistin has been re-introduced into treatment of human MDR bacterial infections. We assessed the characteristics and spread of plasmid-borne colistin resistance among healthy pigs, workers with animal-contact and their household members in Thailand.Methods WGS and MIC data of 146 mcr-positive isolates from a cross-sectional One Health study were analysed. Long-read sequencing and conjugation were performed for selected isolates.Results mcr-carrying isolates were detected in 38% of pooled-pig samples and 16% of human faecal samples. Of 143 Escherichia coli and three Escherichia fergusonii, mcr-1, mcr-3, and mcr-9 variants were identified in 96 (65.8%), 61 (41.8%) and one (0.7%) isolate, respectively. Twelve E. coli co-harboured two mcr variants (mcr-1 and mcr-3). Clonal transmission was detected in five out of 164 farms. mcr-1 was mostly harboured by epidemic IncX4 and IncHI1 plasmids (89.9%). Conversely, mcr-3 was harboured by a range of different plasmids. Comparative plasmid studies suggested IncP and IncFII plasmids as possible endemic mcr-3 plasmids in Asian countries. Moreover, mcr-3 was associated with different mobile genetic elements including TnAs2, ISKpn40 and IS26/15DI. Detected genetic signatures (DRs) indicated recent mcr-3 transpositions, underlining the mobilizable nature of the mcr-3 cassette.Conclusions The epidemiology of mcr and the possible evolution of successful plasmids and transposition modules should be carefully monitored. Of special concern is the growing number of different horizontal gene transferring pathways encompassing various transposable modules the mcr genes can be shared between bacteria.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Leangapichart, T ...
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Stosic, Milan S
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Hickman, Rachel ...
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Lunha, Kamonwan
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Jiwakanon, Jates ...
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Angkititrakul, S ...
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show more...
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Magnusson, Ulf
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Van Boeckel, Tho ...
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Järhult, Josef D ...
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Sunde, Marianne
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
- Articles in the publication
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Journal of Antim ...
- By the university
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Uppsala University