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Observation of a si...
Observation of a single protein by ultrafast X-ray diffraction
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- Ekeberg, Tomas, 1983- (author)
- Uppsala universitet,Molekylär biofysik,Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden, Husargatan 3 (Box 596)
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Assalauova, Dameli (author)
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- Bielecki, Johan (author)
- European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany
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Boll, Rebecca (author)
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- Daurer, Benedikt J. (author)
- Diamond Light Source, Harwell Science & Innovation Campus, Didcot, OX11 0DE, UK
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Eichacker, Lutz A. (author)
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Franken, Linda E. (author)
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Galli, Davide E. (author)
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Gelisio, Luca (author)
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Gumprecht, Lars (author)
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- Gunn, Laura H., 1986- (author)
- Uppsala universitet,Molekylär biofysik,Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA
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- Hajdu, Janos (author)
- Uppsala universitet,Molekylär biofysik
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Hartmann, Robert (author)
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- Hasse, Dirk (author)
- Uppsala universitet,Molekylär biofysik
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Ignatenko, Alexandr (author)
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- Koliyadu, Jayanath C. P. (author)
- KTH,Biomedicinsk fysik och röntgenfysik,European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany, Holzkoppel 4
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Kulyk, Olena (author)
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Kurta, Ruslan (author)
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Kuster, Markus (author)
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Lugmayr, Wolfgang (author)
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Lübke, Jannik (author)
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Mancuso, Adrian P. (author)
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Mazza, Tommaso (author)
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- Nettelblad, Carl, 1985- (author)
- Uppsala universitet,Avdelningen för beräkningsvetenskap,Tillämpad beräkningsvetenskap,Science for Life Laboratory, SciLifeLab,Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden, Husargatan 3 (Box 596); NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA,Molekylär biofysik
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Ovcharenko, Yevheniy (author)
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Rivas, Daniel E. (author)
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Rose, Max (author)
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Samanta, Amit K. (author)
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Schmidt, Philipp (author)
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Sobolev, Egor (author)
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- Timneanu, Nicusor (author)
- Uppsala universitet,Kemisk och biomolekylär fysik
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Usenko, Sergey (author)
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- Westphal, Daniel (author)
- Uppsala universitet,Molekylär biofysik
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Wollweber, Tamme (author)
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Worbs, Lena (author)
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Xavier, Paul Lourdu (author)
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Yousef, Hazem (author)
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Ayyer, Kartik (author)
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Chapman, Henry N. (author)
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- Sellberg, Jonas A., 1985- (author)
- KTH,Biomedicinsk fysik och röntgenfysik
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Seuring, Carolin (author)
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Vartanyants, Ivan A. (author)
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Küpper, Jochen (author)
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Meyer, Michael (author)
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- Maia, Filipe R. N. C. (author)
- Uppsala universitet,Molekylär biofysik,NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA
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(creator_code:org_t)
- Springer Nature, 2024
- 2024
- English.
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In: Light. - : Springer Nature. - 2095-5545 .- 2047-7538. ; 13:1
- Related links:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired many. It was one of the arguments for building X-ray free-electron lasers. According to theory, the extremely intense pulses provide sufficient signal to dispense with using crystals as an amplifier, and the ultrashort pulse duration permits capturing the diffraction data before the sample inevitably explodes. This was first demonstrated on biological samples a decade ago on the giant mimivirus. Since then, a large collaboration has been pushing the limit of the smallest sample that can be imaged. The ability to capture snapshots on the timescale of atomic vibrations, while keeping the sample at room temperature, may allow probing the entire conformational phase space of macromolecules. Here we show the first observation of an X-ray diffraction pattern from a single protein, that of Escherichia coli GroEL which at 14 nm in diameter is the smallest biological sample ever imaged by X-rays, and demonstrate that the concept of diffraction before destruction extends to single proteins. From the pattern, it is possible to determine the approximate orientation of the protein. Our experiment demonstrates the feasibility of ultrafast imaging of single proteins, opening the way to single-molecule time-resolved studies on the femtosecond timescale.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ekeberg, Tomas, ...
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Assalauova, Dame ...
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Bielecki, Johan
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Boll, Rebecca
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Daurer, Benedikt ...
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Eichacker, Lutz ...
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show more...
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Franken, Linda E ...
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Galli, Davide E.
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Gelisio, Luca
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Gumprecht, Lars
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Gunn, Laura H., ...
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Hajdu, Janos
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Hartmann, Robert
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Hasse, Dirk
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Ignatenko, Alexa ...
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Koliyadu, Jayana ...
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Kulyk, Olena
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Kurta, Ruslan
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Kuster, Markus
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Lugmayr, Wolfgan ...
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Lübke, Jannik
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Mancuso, Adrian ...
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Mazza, Tommaso
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Nettelblad, Carl ...
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Ovcharenko, Yevh ...
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Rivas, Daniel E.
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Rose, Max
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Samanta, Amit K.
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Schmidt, Philipp
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Sobolev, Egor
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Timneanu, Nicuso ...
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Usenko, Sergey
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Westphal, Daniel
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Wollweber, Tamme
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Worbs, Lena
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Xavier, Paul Lou ...
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Yousef, Hazem
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Ayyer, Kartik
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Chapman, Henry N ...
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Sellberg, Jonas ...
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Seuring, Carolin
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Vartanyants, Iva ...
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Küpper, Jochen
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Meyer, Michael
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Maia, Filipe R. ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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Uppsala University
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Royal Institute of Technology